... X. Lan Vu for their technical assistance, R. Brun of the Swiss Trop-ical Institute, University of Basel, for T. cruzi cultures,the laboratory of J. H. Hurley (NIH, Bethesda, MD,USA) for providing ... MD,USA) for providing expression plasmids for Vps27p-FYVE-GST fusion proteins, Peter Buetikofer (Institute of Molecular Biology, University of Bern) for provi-ding phospholipids and valuable ... nonconserved). Only six of these substitutions occurin the catalytic domain of the enzyme, and none of them affects a residue that is crucial for function (seebelow). Southern blot analysis of T. cruzi...