Tài liệu Báo cáo khoa học: Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies Alteration of substrate specificity by replacement of Asn149 with Asp docx
... Insights into the interaction of human arginase II with
substrate and manganese ions by site-directed
mutagenesis and kinetic studies
Alteration of substrate specificity by replacement of Asn149
with ... examine the interaction of human arginase II (EC 3.5.3.1) with sub-
strate and manganese ions, the His120Asn, His14...
... PH
domains (B) and the four PLD specific con-
served regions (I–IV) are marked. Regions II
and IV represent the two HKD motifs form-
ing the active site. In (A), the positions of
the 30 tyrosine and 15 ... and poppy [20,21].
In the present study, the first insights into the secon-
dary and tertiary structure of PLD a2 from cabbage
are given. The surface str...
... fact, the bulk
of the proteolytic processing of the proforms of the
SVMPs occurs in advance of release of mature secre-
tory vesicle contents into the lumen of the venom gland.
In the case of the ... domains of these two proteins, although the C-ter-
minal regions of the domains are probably similar to
those of the rest of the class. Whether th...
... is
a chunk and the edge represents the relations be-
tween the connected nodes labeled with the karaka
or other relations. All the modifier-modified rela-
tions between the heads of the chunks ... acyclicity and the sin-
gle head constraints, which impose restrictions
on the dependency relation as such, projectivity
constrains the interaction between the dependen...
... polysaccharide,
pullulan.
Comparison of the active sites of the model of IPU
and Dex49A
The active site structures of t he model of IPU (Fig. 4A) and
Dex49A (Fig. 4B) were compared. The three Asp residues,
Asp3 53, Asp3 72 and Asp3 73, ... 20–564), and the model is
composed of residues 25–540. The overall structure of the
model of IPU and the mut...
... coordination interaction with one of the manganese
ions. This, together with the fact that the positions of other
Fig. 4. A superimposition of the structures of B. caldovelox arginase
(red), rat liver arginase ... the sequences of 1CEV and 1RLA and pasted into the
structural alignment. The alignment was then corrected by
hand. Since the sequences...
... addition of ISP and Qcr10p).
On the other hand, in the transition from the 500 kDa
band to the 670 kDa band, a structural rearrangement
of the bc
1
complex may occur due to the binding of ISP
and ... specifically
required for the insertion of ISP into an immature bc
1
complex and that the association of the Qcr9p subunit
with the bc
1
complex precedes t...
... the
lack of domain B protruding out of the TIM barrel in
the place of loop 3 connecting the b3-strand to the
a3-helix [9,21]. Sharing domain B by rBATs and
GH13 enzymes, and especially the sequence of ... oligo-1,6-glucosidase)
in region II of the b4-strand; and (c) more or less the
entire region VII, i.e. the b8-strand. The fact that
rBATs exhibit more...
... decreased the expression levels of HO-2
mRNA and protein in Jurkat T-lymphocytes and
YN-1 and K562 erythroid cell lines.
To further analyze the effects of hypoxia on the
expression of HO-1 and HO-2, ... apart. We therefore analyzed the
promoter function of the 1.5 kb 5¢-flanking region of
the HO-2 gene in the present study.
YN-1 cells were transfected with eac...
... To compare sentences of
different lengths, the joint probability of the whole
sentence and tags was divided by the number of
words in the sentence. The result showed that
the average probability ... predictions
of garden-path effects and the subject/object rela-
tive asymmetry. These previous studies have used
small numbers of examples of, for example, the
R...