... Cloning, characterization and expression analysis of interleukin-10 from the common carp, Cyprinus carpio L. Ram Savan1, Daisuke Igawa2 and Masahiro Sakai21United Graduate School of ... time-points of incubation, exceptfor the 0 h control. The highest level of expression was seenat 1 h post-LPS stimulation of liver cells.DiscussionIL-10, a helical cytokine, was initially isolated ... conditionsAll PCR reactions were performed according to the following protocol: 1 lL of cDNA was mixed with 5 lL of dNTPs (10 l M of each dNTP), 10 · Gene Taq Universalbuffer, 0.5 lLofTaq polymerase...