... MINIREVIEW
Second messenger function and the structure–activity
relationship of cyclic adenosine diphosphoribose (cADPR)
Andreas H. Guse
University Medical Center Hamburg-Eppendorf, Center of Experimental ... between the base adenine
(or hypoxanthine) and the diphospho-bridge, as they
can be replaced by much simpler ether strands. These
ether strands mimic t...
... thetase to esterify the 13cROL generated in the reac-
tion. Therefore, 13cROL is accumulated in the absence
of LRAT in the reaction (Fig. S3B).
Under the in vitro assay conditions of the ... the area of
the 11cROL and 13cROL peaks and expressed as the
mean ± SD of three independent measurements.
Retinal cell dissociation
The zebrafish eyes were enucleated, and...
... 158(21)
and 296(154) [14–17]. The high specific activity of these
FVIIa variants is presumably linked to a more stable
burial of the N-terminus of the protease domain in the
activation pocket of the ... catalytic
efficiency, the overall functional defect of G372A-
FVIIa was, if anything, smaller in the presence of TF,
which suggests that the allosteric effect of...
... presenting the pulse of Src activity in the plane
of the inactive and active fractions, s
i
and s
1
. If the
duration of the stimulus exceeds the critical value, the
trajectory in the (s
i
, ... conformation, the
SH2 domain binds to pY
i
on the C-terminal tail, and
the Src homology 3 (SH3) domain binds to the linker
between the SH2 and kinase domains at...
... structured
within the plane of the membrane. Initial proteomics on the outer mem-
brane from yeast mitochondria suggest that while most of the protein
components are integral in the membrane, most of these ... related proteins from other
fungi, or even in the fission yeast Schizosaccharomyces
pombe. Both the lack of obvious orthologs in other
fungi and the abundance...
... has
Fig. 5. Analysis of the enzymatic activity of His-CoaB R206Q and A276V. The synthesis of 4¢-phosphopantothenoyl-CMP (–cysteine, upper part of
the figure) and of 4¢-phosphopantothenoylcysteine (+ ... from
D
-pantothenate. The Dfp flavoprotein catalyzes the synthe-
sis of the coenzyme A precursor 4¢-phosphopantetheine
from 4¢-phosphopantothenate and cysteine usin...
... As
MgP is also the product of the magnesium chelatase
reaction, this emphasizes the importance of quantita-
tive studies not only of the methyltransferase and
chelatase, but also of the interaction ... stimulated. The discovery that another pro-
tein, Gun4, can both stimulate Mg chelatase and bind
MgP [12,25] adds another layer of complexity to both
the regulation...
... entry of fatty acid into mitochon-
dria. Thus, the steady-state level of malonyl-CoA in
these tissues is determined by the relative activities of
ACC and malonyl-CoA decarboxylase.
Role of the ... ghrelin
and adiponectin alter hypothalamic AMPK and malo-
nyl-CoA levels [13,23,28,30–33]. The genetic evidence
for the role of AMPK in the hypothalamus is less clear
bec...
... genotype-directed
therapy in this disease.
KRAS mutation as a mechanism of
resistance to EGFR-targeted therapy
The KRAS protein is localized to the inner surface of
the cell membrane. The binding of ligand ... reposition-
ing of critical residues surrounding the
ATP-binding cleft of the tyrosine kinase
domain of the receptor and thereby stabilize
the interaction...
... molecule takes the position of
the adenine ring of ATP. The carbon and
oxygen atoms of pantoate are shown in
yellow and red, respectively. The carbon
and oxygen atoms of ATP are shown in red,
the nitrogen ... between
the O2 atom of pantoate and the Nf atom of the side
chain of Lys39. However, there are a large number of
water-mediated networked interact...