... enthalpy is the sum of twoterms: the reaction enthalpy, independent of the bufferused in the experiment, and another term representing the contribution of the proton ionization of the buffer,which ... HCl, 1 mM MgCl2, 100 mM NaCl. The upper panels show the raw data for the heat effect during the titrations; the lowerpanels are the binding isotherms.Table 2. Thermodynamic parameters of NarG(1–15) ... hypothesize that the proton-ated state of the chaperone initiates this cycle, whereas the deprotonated state occurs upon completion of the maturation process of the partner. The nature of the signal...