Tài liệu Báo cáo khoa học: Endogenous expression and protein kinase A-dependent phosphorylation of the guanine nucleotide exchange factor Ras-GRF1 in human embryonic kidney 293 cells pptx

... 2304–2316 ª 2005 FEBS Endogenous expression and protein kinase A-dependent phosphorylation of the guanine nucleotide exchange factor Ras-GRF1 in human embryonic kidney 293 cells Jens Henrik Norum1, ... report the endogenous expres-sion of Ras-GRF1 in HEK293 cells. Serotonin stimulation of HEK293 cells transiently expressing Gs-coupled 5-HT7receptors induced protein kinase A-dependent phosphorylation ... endogenous expression of severalisoforms of the guanine nucleotide exchange factor Ras-GRF1 in HEK293 cells. Serotonin treatment of HEK293 cells, transiently transfected with the Gs-cou-pled...

Tài liệu Báo cáo khoa học: Tissue expression and biochemical characterization of human 2-amino 3-carboxymuconate 6-semialdehyde decarboxylase, a key enzyme in tryptophan catabolism pptx

... restored the bacterial protein [21,33].Interestingly, the same difﬁculty in stripping off the metallocofactor from the protein has been describedfor murine adenosine deaminase, a member of the amidohydrolase ... structures of human and bacterialACMSD, demonstrating that the region surroundingresidues involved in catalysis is more hydrophobic in the human, rather than in the bacterial protein. Finally, ... cerebral malaria and in the brain of a murine model of the syndrome[18,19]. Interestingly, picolinate is reportedly able toprevent the neurotoxic effects of quinolinate in the ratcentral nervous...

Tài liệu Báo cáo khoa học: Functional expression and mutational analysis of ﬂavonol synthase from Citrus unshiu pptx

... substitution of glycine residues by alanine or proline and the exchange of proline by glycine. Glycine residues are frequently found at the C-terminal ends of a helices providing the conforma-tional ... activity The enzymatic activity of the recombinant protein wasexamined in FLS incubations employing dihydroquercetinor dihydrokaempferol as a substrate. Both these ﬂavanolswere accepted, and the ... functions in many plants beyond the scope of pigmentation and ultraviolet screening, e.g. in reproduction [1], in the defense against microbial pathogens and insects or in auxin transport [2], and...

Tài liệu Báo cáo khoa học: Mixed lineage leukemia: a structure–function perspective of the MLL1 protein ppt

... protein (CBP) [23], a peptide from the Win motif of MLL1bound to the WD40 repeat protein, WDR5 [24,25] and the C-terminal SET domain in the presence and absence of histone peptides and the cofactor ... coordinates two zinc ions using the two conservedCGXCXXC motifs (Fig. 2A). The zinc ions are requiredfor the structural integrity of the protein, as mutation of any of the cysteine residues involved ... ligands(Fig. 1B). Structures that have been determined include the MLL1 CXXC domain [22], a portion of the MLL1TAD bound to the KIX domain of the cAMPresponse element-binding (CREB) binding protein (CBP)...

Tài liệu Báo cáo khóa học: Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation 2 process of onconase pdf

... was examined on K562 human erythroleukemic cells and A431 human epidermoid carcinoma cells by measuring the incorporation of [35S]methionine into newly synthesizedproteins after 96 h of incubation ... simultaneouslyinteracts with the main phosphate of the substrate [32]. The closer positioning of the N-terminus to the main protein body is essential for the activity and, together with the 87–104 ... N-terminus of the protein wasconﬁrmed by protein sequence determination, which resul-ted in no phenylthiohydantoin derivatives, indicating ablocked N-terminus. Protein identity and integrity...

Tài liệu Báo cáo khoa học: Steady-state and time-resolved ﬂuorescence studies of conformational changes induced by cyclic AMP and DNA binding to cyclic AMP receptor protein from Escherichia coli ppt

... 18.7 A˚onbinding of cAMP to the anti-cAMP-binding sites of the protein. The distance between the sulfur atom of Cys178 and the C9–C10 bond of the indole ring of Trp85, derived from the crystal ... absence of cAMP in apo-CRP, some b strands of the N-terminal domain of the protein may collapse into the cAMP-binding pocket, causing reorientation of the smaller domain in relation to the larger ... and suggest that the binding of cAMP to anti-cAMP-bindingsites of CRP leads to the increase in the structuraldynamic motion of at least Trp13, which is located in the N-terminal domain of the...

Tài liệu Báo cáo khoa học: "ENGLISH WORDS AND DATA BASES: HOW TO BRIDGE THE GAP" pdf

... of the input language and the primitives of the data base, which makes it possible to translate input questions into query expressions in a rather straightforward fashion. In designing the ... independently of each other (one on the basis of the possible Eng- lish questions about the subject-domain, the other on the basis of the structure of the data base about it) there must be ... levels of semantic analysis are distinguished in the PHLIQAI program. At each of these levels, the meaning of the input question is represented by an expression of a formal logical language. The...

Tài liệu Báo cáo khoa học: Homologous expression of the nrdF gene of Corynebacterium ammoniagenes strain ATCC 6872 generates a manganese-metallocofactor (R2F) and a stable tyrosyl radical (Y•) involved in ribonucleotide reduction ppt

... [35] may not be considered in the light of the results obtained in the present study. Rather, the involvement of manganese in the in vivo assembly of the metallocofactor of C. ammoniagenes R2F is ... 37 °C and thenplated for selection of transformants. Protein techniques Protein was determined by protein- dye binding with BSA asa standard [47]. Whole cell protein of C. ammoniagenes cells was ... of protein uti-lizing the principle of protein- dye binding. Anal Bio-chem 72, 248–254.48 Laemmli UK (1970) Cleavage of structural proteinsduring the assembly of the head of bacteriophage T4.Nature...

Tài liệu Báo cáo khoa học: Differential expression of duplicated LDH-A genes during temperature acclimation of weatherﬁsh Misgurnus fossilis Functional consequences for the enzyme ppt

... located in the sub-unit contact area of the tetramer, whereas the remaining two mismatches sur-round the contact area; this is expected to manifest in the kinetic and thermodynamic properties of the ... different kinetic and thermodynamic properties of the tetrameric enzymemay result in ﬁne-tuning of the properties of skeletal LDH-A, which is in linewith previously observed kinetic and thermodynamic ... carry in their 3¢-UTR sequence bindingsites for speciﬁc proteins that increase ⁄ decrease the rate of poly(A) shortening, i.e. affect the lifetime of the mRNA [32], therefore it is likely that the...

Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx

... coordinatedchanges in the expression of the transferrin receptor and ferritin; (b) developed iron accumulation and (c) increased the prooxidant state and the level of oxidatively modiﬁed proteins. ... in the cytoplasm of human epithelial cells of the gastrointestinal tract and liver, two central organs in the regulation of ironmetabolism [20,21]. After their synthesis in the endo-plasmic ... 161Determination of phosphatidylserine expression and cell deathPhosphatidylserine expression on the outer part of the plasma membrane was examined by Annexin V binding.Brieﬂy, cells were...

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