Tài liệu Báo cáo khoa học: The RNA recognition motif, a plastic RNA-binding platform to regulate post-transcriptional gene expression ppt

... 12,5191–5200. The RRM domain, a plastic RNA- binding platform C. Maris et al.2130 FEBS Journal 272 (2005) 2118–2131 ª 2005 FEBSMINIREVIEW The RNA recognition motif, a plastic RNA- binding platform to regulate ... clear whythese RRM domains that are very similar to the classical ones favor interaction with proteins ratherthan RNA. Conclusion and perspectives The RNA recognition motif is an abundant and ... for the protein–proteininteraction are displayed as balls and sticks.This ﬁgure was generated using the pro-gramsMOLSCRIPT and RASTER3D [57,58].C. Maris et al. The RRM domain, a plastic RNA- binding...

Tài liệu Báo cáo khoa học: The capsid protein of human immunodeﬁciency virus: designing inhibitors of capsid assembly ppt

... privateFoundation (Exp: 36557⁄ 06) and Generalitat Valenci-ana (ACOMP ⁄ 2009⁄ 185). Drs Mauricio G. Mateu andFrancisco N. Barrera are thanked for critically reading the manuscript and for their ... del Carmen Lidon-Moya and Estefanı´ a Hurtado-Go´mez are acknowledged for their careful experimentalwork. May Garcı´ a, Marı´ a del Carmen Fuster, JavierCasanova and Olga Ruiz de los Pan˜os ... collaboration over allthese years. Dr Anjali P. Mascarenhas and ProfessorKarin Musier-Forsyth are thanked for critically read-ing and correcting an early version of the manuscript.Marı´a...

Tài liệu Báo cáo khoa học: The stereochemistry of benzo[a]pyrene-2¢-deoxyguanosine adducts affects DNA methylation by SssI and HhaI DNA methyltransferases pptx

... benzo [a] pyrene-2¢-deoxyguanosineadducts affects DNA methylation by SssI and HhaI DNAmethyltransferasesOksana M. Subach1, Diana V. Maltseva1, Anant Shastry2, Alexander Kolbanovskiy2,Saulius ... (+)-trans-B [a] P-N2-dG.GCGref5¢-GAGCCAAGCGCACTCTGACGMref5¢-TCAGAGTGMGCTTGGCTCGCG 5¢-CACCCTTGCGCTCTCTCACGC 5¢-TGAGAGAGCGCAAGGGTGCGM 5¢-TGAGAGAGMGCAAGGGTGX+CG 5¢-CACCCTTX+CGCTCTCTCAGCX+5¢-CACCCTTGCX+CTCTCTCAY+CG ... B [a] P-N2-dG adducts was observed [19]. The B [a] P-DNA adducts also affect the function of humantopoisomerase I by alteration of DNA cleavage patterns[20]. The greatest disturbance of DNA cleavage...

Tài liệu Báo cáo khoa học: The nuclear lamina Both a structural framework and a platform for genome organization pdf

... family The nuclear lamina is a complex ensemble of proteinsthat connects the inner nuclear membrane to chroma-tin, and thus creates a link from the cytoplasm to the genome. The nuclear lamina has ... proteins and chromatinassociating factors. The constituent proteins, the lamins, have recently gainedtremendous recognition, because mutations in the lamin A gene, LMNA, are the cause of a complex ... AM, Columbaro M, Scarano G, MattioliE, Sabatelli P, et al. (2005) Alterations of nuclear envel-ope and chromatin organization in mandibuloacral dys-plasia, a rare form of laminopathy. Physiol...

Tài liệu Báo cáo khoa học: The lipid ⁄ protein interface as xenobiotic target site Kinetic analysis of tadpole narcosis ppt

... subunitmutations are both sensitive and resistant to volatileanesthetics. Anesth Analges 95, 578–582.38 Takasaki M, Tatara T, Suezaki Y, Shirahama K,Kamaya H, Ueda I & Totoki T (1991) Effect of inhala-tion ... molecular targetsare most relevant to general anaesthesia? Toxicol Lett100–101, 1–8.17 Mihic SJ, Ye Q, Wick MJ et al. (1997) Sites of alcoholand volatile anaesthetic action on GABA A and glycinereceptors. ... to the data points for tadpole narcosisby the additional alcohols of Table 1 are shown inFig. 2 along with the theoretical curves for the enhancement of agonist binding to the nAChR. The latter...

Tài liệu Báo cáo khoa học: "The impact of language models and loss functions on repair disﬂuency detection" pptx

... the reparandum and the repair. Example (3)shows the crossing dependencies. As this exam-ple also shows, the repair often contains many of the same words that appear in the reparandum. Infact, ... Y].∂E[e]/∂wjis given by a similar formula.9 ResultsWe follow Charniak and Johnson (2001) and split the corpus into main training data, held-out train-ing data and test data as follows: main training con-sisted ... .Noisy-channel models are used in a similar wayin statistical speech recognition and machine trans-lation. The language model assigns a probabilityP (X) to the string X, which is a substring of the observed...

Tài liệu Báo cáo khoa học: "Using Error-Correcting Output Codes with Model-Refinement to Boost Centroid Text Classifier" ppt

... so-called bias. The basic idea is to take advantage of misclassified examples in the training data to iteratively refine and adjust the centroids of text data. The experimental results reveal ... classifier using the binary class (0 and 1) over the total training data. TESTING 1 Apply each of the L classifiers to the test example. 2 Assign the test example the class with the largest ... problems (Ghani 2000). Any learning algorithm that can handle two-class learning problems, such as Naïve Bayes (Sebastiani 2002), can then be applied to learn each of these L problems. L can then...

Tài liệu Báo cáo khoa học: The double-stranded RNA-binding motif, a versatile macromolecular docking platform doc

... of the RNAi ⁄ miRNA pathway, whichcarry an RNase domain for dsRNA cleavage and pro-cessing [12–14]. A second group of dsRBM-containing proteins donot carry an identiﬁable catalytic domain. ... interactions, thusadding an additional regulatory layer to dsRBM RNA recognition. Interaction with non-dsRNA partnersAlthough dsRNA-binding is the deﬁning feature ofdsRBMs, other macromolecular ... such as the onesfound in PKR and RHA can regulate catalytic activitywithin the same protein. PKR is a vital component of the cellular antiviral mechanism and binding to dsRNA (synthesized in large...

Tài liệu Báo cáo khoa học: The tandemly repeated domains of a b-propeller phytase act synergistically to increase catalytic efﬁciency doc

... ⁄PAGE.Puriﬁcation was performed at 4 °C. The supernatantswere concentrated through a hollow ﬁber cartridge (cutoff6 kDa; Motianmo, Tianjin, China) and the His-taggedproteins in the supernatants were adsorbed ... Protein concentration wasdetermined using the Bradford assay with BSA as the standard [28].Phytase activity assayPhytase activity was determined by measuring the amountof phosphate released from ... determination of the phytase gene Strain HJB17 was cultured in Luria–Bertani medium at37 °C overnight and genomic DNA was extracted using the TIANamp Bacteria DNA kit (Tiangen, Beijing, China).The...

Tài liệu Báo cáo khoa học: Crystal structure of importin-a bound to a peptide bearing the nuclear localisation signal from chloride intracellular channel protein 4 ppt

... in an extended conformation.DatabaseStructural data are available in the protein Data Bank under the accession number3OQS.Structured digital abstractlCLIC4 and importin alpha bind by x-ray ... The CLIC4 NLS binds in anextended conformation that runs antiparallel to impor-tin -a, analogous with other NLS cargo. The averageatomic B factors for importin -a in the structure are32.1 A ˚2for ... main-chain atoms, 35.7 A ˚2for side-chainatoms and 33.8 A ˚2overall (3244 atoms). For the pep-tide, B factors are slightly higher than those forimportin -a: 36.9 A ˚2for main-chain atoms,...

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