Tài liệu Báo cáo khoa học: Structural characterization of Ca2+/CaM in complex with the phosphorylase kinase PhK5 peptide pdf

... substratebinding sites. In the case of the titin and twitchinkinases, the autoinhibitory sequence acts as a pseudo-substrate, occluding ATP binding and preventingprotein substrates from binding ... CaM. However in PhK5 Trp357 is foundat the C-terminal end of the peptide. This suggeststhat either PhK5 might bind with Trp357 in the N-terminal site in CaM, or perhaps could bind in the opposite ... protein kinases are regulated throughan autoinhibitory mechanism [8]. In these structures aC-terminal extension to the protein kinase folds backon the kinase domain and interferes with the...

Tài liệu Báo cáo khoa học: Structural features of proinsulin C-peptide oligomeric and amyloid states pptx

... structural role in the proper foldingand disulﬁde bonding in insulin [2]. After proinsulincleavage, it is released to the blood together with insu-lin in equimolar amounts. The 31-residue peptide ... distribu-tion of 100 lM proinsulin C -peptide in the presence of divalent Ca2+and Mg2+ionsunder native conditions (B), and of 100 lMC -peptide in the presence of insulin (C) and of 100 lM C -peptide ... M(2001) Proinsulin C -peptide rapidly stimulates mitogen-activated protein kinases in Swiss 3T3 ﬁbroblasts:requirement of protein kinase C, phosphoinositide3 -kinase and pertussis toxin-sensitive...

Tài liệu Báo cáo khoa học: Molecular characterization of Arabidopsis thaliana PUF proteins – binding speciﬁcity and target candidates doc

... through the binding of RNA-binding proteins to 3¢ UTR tran-scripts has been poorly described in plants.PUF proteins are a large family of RNA-bindingproteins found in all eukaryotes. These proteins ... Scheme of the three-hybrid strategy used in the screen. (B) Number of colonies identiﬁed in each step of the screen. (C) Distribution of the 63 distinct sequences in relation of their position in the ... 5E).These data suggest that binding afﬁnity may be in u-enced either by nucleotides outside of the consensusmotif or by small variations within the consensus. The interaction of APUM-2 with the...

Tài liệu Báo cáo khoa học: Functional characterization of an orphan cupin protein from Burkholderia xenovorans reveals a mononuclear nonheme Fe2+-dependent oxygenase that cleaves b-diketones ppt

... byxanthine oxidase was followed indirectly by measuring the increase in absorbance at 550 nm resulting from cyto-chrome c being reduced by superoxide in the course of the reaction. The principle of ... Interestingly, the position of the glutamate ligand was not conserved in the aminoacid sequence, relative to the cupin core motifs thatcontribute the three histidine ligands in each of theseproteins.For ... rateand [E] is the molar concentration of the 16 kDaprotein subunit) increased linearly with increasingfractional saturation of the metal site in Bxe_A2876, the kcatdetermined from the molar concentration...

Tài liệu Báo cáo khoa học: Structural effects of a dimer interface mutation on catalytic activity of triosephosphate isomerase The role of conserved residues and complementary mutations pptx

... only criticalfor the stability of the dimer, it may also be involved in maintaining the integrity of the active site. These resultsclearly suggest that the dimer interface in the Y74W*mutant ... the backbone CO of F102. Fur-thermore, the orientation of the side chain of the tworesidues brings the guanidinium plane and the aro-matic ring of F102 into close proximity, with analmost ... R98 is involved in key interactions with T75 across the dimer interface,while T75 interacts with N10 and E97 of the secondsubunit. The backbone NH group of R98 forms ahydrogen bond with the backbone...

Tài liệu Báo cáo khoa học: Kinetic characterization of the ﬁrst step of the ribozyme-catalyzed trans excision-splicing reaction docx

... to the GBS of the ribozyme for the exon-ligation step of TES. In the substrate-cleavagestep, however, the 3¢ terminal guanosine (G336) of the ribozyme (Fig. 1) is binding to that same GBS.Therefore, ... for the second step of TES [48], similar to (if not the same as) the role of the xG in the second step of self-splicing [48–56].This suggests that the guanosine to be excised islikely binding ... not the limitingstep (binding is slower), but in terms of decreasing the amount of substrate that dissociates from the ribozymebefore reactivity, effectively increasing the yield of the reaction.Experimental...

Tài liệu Báo cáo khoa học: Spectroscopic characterization of a higher plant heme oxygenase isoform-1 from Glycine max (soybean) ) coordination structure of the heme complex and catabolism of heme docx

... nm of each free hemin solution was measured, and these wereplotted together with that of the corresponding hemin–protein solutions. The equivalent was determined at an in ection point of the ... intersectionpoint corresponding to the mole fraction of 0.5 of the respective forms gives the amount of azide necessary toattain the equilibrium, thereby giving the equilibrium con-stant for azide binding, ... useful information on the heme pocketstructure. As shown in Table 2, the hyperﬁne splittingconstants of the 15N nucleus of the distal NO and of the 14N nucleus of the proximal His of the GmHO-1complex...

Tài liệu Báo cáo khoa học: Thermodynamic characterization of interleukin-8 monomer binding to CXCR1 receptor N-terminal domain ppt

... November2006)doi:10.1111/j.1742-4658.2006.05579.xChemokines elicit their function by binding receptors of the G-protein-cou-pled receptor class, and the N-terminal domain (N-domain) of the receptoris one of the two critical ligand-binding sites. ... in uencedby the choice of buffer if proton transfer accompanies the binding process. In this case, the measured enthalpy of binding is linearly related to the intrinsic ionizationenthalpy of ... forunderstanding the relationship between structure and the thermodynamics of binding.IL-8 binds the isolated N-domain with an afﬁnitysimilar to that for the N-domain in the intact receptor,and...

Tài liệu Báo cáo khoa học: Biochemical characterization of human 3-methylglutaconyl-CoA hydratase and its role in leucine metabolism docx

... usingcrotonyl-CoA as a substrate [15]. The gene encodingthis bifunctional protein was named AUH (‘AU bind-ing homolog of enoyl-CoA hydratase’). The RNA-binding activity of the human protein ... to an HPLC vial. The products of the reaction were detected at 260 nm using the HPLC system described above for the synthesis of the CoA-esters. The assay was linear with an incubation time up ... factor of 20 over the dehydration reac-tion (kcat¼ 0.26) which is consistent with the mainrole of AUH in leucine catabolism, the hydration of 3-MG-CoA. Comparing the turnover numbers of glu-taconyl-CoA...

Tài liệu Báo cáo khoa học: Biochemical characterization of Bacillus subtilis type II isopentenyl diphosphate isomerase, and phylogenetic distribution of isoprenoid biosynthesis pathways doc

... subtilisprotein) in the direct vicinity of the FMN binding site whichis not present in the other members of the family.Thus, under structural aspects, Idi-2 appears as a fairlydistant relative of the ... of the enzyme for that cofactor and the apparent absence of a redox process as part of the catalyticcycle. The substrate binding site of the type II enzymeremains veiled. However, a patch of ... beinvolved in the binding of the FMN cofactor [29] areabsolutely conserved (Fig. 2A,B). These residues (markedby ﬁlled triangles in Fig. 2A) are located in four differentsegments of the peptide chain....

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