Tài liệu Báo cáo khoa học: N-Methyl-L-amino acid dehydrogenase from Pseudomonas putida A novel member of an unusual NAD(P)-dependent oxidoreductase superfamily ppt

... N-Methyl-L-amino acid dehydrogenase from Pseudomonas putida A novel member of an unusual NAD(P)-dependent oxidoreductase superfamily Hisaaki Mihara1,*, Hisashi Muramatsu1,*, Ryo Kakutani1, ... were of analytical grade from Nacalai Tesque (Kyoto, Japan) andWako Pure Chemical Industries (Osaka, Japan).Culture and screening of bacteriaBacterial strains were cultivated for 15–20 h at ... enolase, lactate dehydrogenase, andglutamate dehydrogenase from Oriental Yeast, Osaka,Japan.Other analytical methodsThe N-terminal amino -acid sequence of the enzyme wasdetermined with an automated...

Tài liệu Báo cáo khoa học: Amino acid discrimination by arginyl-tRNA synthetases as revealed by an examination of natural speciﬁcity variants doc

... Preparation and colorimetricanalysis of l-canavanine. Anal Biochem 77, 147–151.60 Ozinskas AJ & Rosenthal GA (1986) l-Canavanine syn-thesis by zinc-mediated guanidination of l-canaline ... Comparison of canavanine incorporation. The amount of L-canavanine transferred by arginyl-tRNA synthetase from E. coli(waved shading), jack bean (vertical dashes) and soybean (diagonalshading) ... 5. Stability of canavanyl-tRNA. Jack bean transcript tRNAArgthat had been aminoacylated with [14C]-L-canavanine was incubatedin the absence of enzyme (), or in the presence of jack bean(d)...

Tài liệu Báo cáo khoa học: Fatty acid desaturases from the microalga Thalassiosira pseudonana pptx

... chain base; LCPUFA, long chain polyunsaturated fatty acid; PUFAs, polyunsaturated fatty acids;RACE, rapid ampliﬁcation of cDNA ends; TAG, triacyglycerol; Tpdes, Thalassiosira pseudonana desaturase.FEBS ... Qing R, Harvey D, Li Y, Larson TR &Graham IA (2005) Identiﬁcation of a long-chain poly-unsaturated fatty acid acyl-coenzyme A synthetase from the diatom Thalassiosira pseudonana. Plant Physiol ... substrates.AbbreviationsARA, arachidonic acid; DHA, docosahexaenoic acid; EPA, eicosapentaenoic acid; EST, expressed sequence tag; FAME, fatty acid methylester; gDNA, genomic DNA; LCB, long chain...

Tài liệu Báo cáo khoa học: Hypoxic resistance to articular chondrocyte apoptosis – a possible mechanism of maintaining homeostasis of normal articular cartilage pdf

... examination of thearticular cartilage after experimentally inducedOAArticular cartilage of the femoral condyles from theexperimental joints was examined to assess any macro-scopic damage ... chondrocytes,western blot analysis was performed. It was shownthat cotreatment of cells with ALLN and TRAILincreased caspase-8 activation and JNK phosphoryla-tion. However, both caspase-8 activation and JNKphosphorylation ... progression of inflammation and may be detected in the synovialfluid. These changes could be associated with a naturalhistory of OA and may be beneficial in the detection of patients at risk of rapidly...

Tài liệu Báo cáo khóa học: UDPgalactose 4-epimerase from Saccharomyces cerevisiae A bifunctional enzyme with aldose 1-epimerase activity docx

... SDS/PAGE analysis at each step of purification.Lane 1, crude extract (cytosol); lane 2, ammonium sulfate precipitatedand Amicon ultramembrane filtered fraction; lane 3, pooled fraction from DEAE-Sephacel ... Sequence analysis indicates thatthe yeast epimerase has an N-terminal domain (residues1–377) that shows significant similarity with Escherichia coliand human UDPgalactose 4-epimerase, and a C-terminaldomain ... formation of NADH was measured at 340 nm over a linear range of 2–5 min.Mutarotase assayMutarotase activity was measured with a DIP-360 polari-meter (Jasco). This assay is based upon the change in...

Tài liệu Báo cáo khoa học: Fatty acid synthesis Role of active site histidines and lysine in Cys-His-His-type b-ketoacyl-acyl carrier protein synthases ppt

... is an importantfactor for efﬁcient transacylation activity, especially at4 °C.Elongation activity of the wild-type and K328mutant KAS I proteinsThe transfer and decarboxylation partial reactionscharacterizing ... CGATTACCTGAACTCCGAGGGTACTTCGACTCCGH298Q, CGATTACCTGAACTCCCAGGGTACTTCGAGTCCGK328H, (5¢-GGCGATTTCTGCAACCCACGCCATGACCGGTCAC-3¢)K328R, (5¢-GGCGATTTCTGCAACCCGTGCCATGACCGGTCAC-3¢)K328E, (5¢-GGCGATTTCTGCAACCGAAGCCATGACCGGTCAC-3¢)K328I, ... hypothesis, as triace-tic acid lactone cannot breakdown to give acetyl-ACP.Either the acetyl carbanion has a signiﬁcant lifetime inthe absence of an acyl acceptor or it is transferred to an unknown,...

Tài liệu Báo cáo khoa học: The SCO2299 gene from Streptomyces coelicolor A3(2) encodes a bifunctional enzyme consisting of an RNase H domain and an acid phosphatase domain pdf

... 88,12–19.19 Ohtani N, Yanagawa H, Tomita M & Itaya M (2004)Identiﬁcation of the ﬁrst archaeal type 1 RNase H gene from Halobacterium sp. NRC-1: archaeal RNase HI cancleave an RNA-DNA junction. ... enzyme consisting of an RNase Hdomain and an acid phosphatase domainNaoto Ohtani1, Natsumi Saito1, Masaru Tomita1, Mitsuhiro Itaya1,2and Aya Itoh11 Institute for Advanced Biosciences, ... [19,20]. ArchaealType 1 RNase H can cleave an RNA–DNA junction (a junction between the 3¢ side of RNA and 5¢ side of DNA) of an Okazaki fragment-like substrate (RNA9–DNAÆDNA), unlike other cellular...

Tài liệu Báo cáo khoa học: EGF receptor in relation to tumor development: molecular basis of responsiveness of cancer cells to EGFR-targeting tyrosine kinase inhibitors docx

... signal-ing by binding and antagonizing the anti-apoptoticBcl-2 family members, thereby causing activation of Bax and Bak [23]. Regulation of Bcl-2 family memberscan occur by a number of mechanisms, ... irradiation inhuman keratinocytes results in induction of a confor-mational change in Bax and its translocation to mito-chondria [63], p38 may be an important upstreammolecule of Bax activation ... extracellular ligand-binding domain, a transmembrane domain and an intracellular tyrosinekinase domain. The binding of a ligand to the extracel-lular domain of the EGFR induces receptor dimeriza-tion,...

Tài liệu Báo cáo khoa học: Pronounced adipogenesis and increased insulin sensitivity caused by overproduction of prostaglandin D2 in vivo pptx

... 5¢-GGAGATCTCCAGTGATATCGACCA-3¢ and 5¢-ACGGCTTCTACGGATCGAAACT-3¢ for PPARc ,5¢-AAGACAGCTCCTCCTCGAAGGTT-3¢ and 5¢-TGACCAAATCCCCATTTACGC-3¢ for aP2,5¢-ATCCATGGATGGACGGTAACG-3¢ and 5¢-CTGGATCCCAATACTTCGACCA-3¢ ... 5¢-GCGTCGGGTAGATCCAGTT-3¢ and 5¢-CTCAGTGGGGCTTAGCTCTG-3¢ for ACC, and 5¢-AACACCCCAGCCATGTACGTAG-3¢ and 5¢-TGTCAAAGAAAGGGTGTAAAACGC-3¢ for b-actin. Expression levels of the target genes were normalized to that of ... scanner (LaTheta LCT-100; Aloka, Tokyo,Japan). Data were analyzed using latheta software (Alo-ka). The fat and muscle weights were determined from an image at the level of the umbilicus. Subcutaneous...

Tài liệu Báo cáo khoa học: Neuronal growth-inhibitory factor (metallothionein-3): a unique metalloprotein ppt

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