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Tài liệu Báo cáo khoa học: Molecular characterization, phylogenetic relationships, and developmental expression patterns of prion genes in zebrafish (Danio rerio) doc

Tài liệu Báo cáo khoa học: Molecular characterization, phylogenetic relationships, and developmental expression patterns of prion genes in zebrafish (Danio rerio) doc

Tài liệu Báo cáo khoa học: Molecular characterization, phylogenetic relationships, and developmental expression patterns of prion genes in zebrafish (Danio rerio) doc

... mammalian origin by the fish intestine.Comparison of zebrafish prp1, prp2, and prp3 developmental expression patterns The developmental expression patterns of prp1 and prp2 coding for long zebrafish ... 2004 FEBS 513 Molecular characterization, phylogenetic relationships, and developmental expression patterns of prion genes in zebrafish (Danio rerio) Emmanuelle Cotto1,2, Miche`le Andre´1, ... a member of the PrP family.Analysis of the N-terminal repeat domain of zebrafish PrP1 and PrP2The N-terminal domain of zebrafish PrP1 containednine repeats (residues 53–332) including four highlyconserved...
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Tài liệu Báo cáo khoa học: Molecular characterization of Arabidopsis thaliana PUF proteins – binding specificity and target candidates doc

Tài liệu Báo cáo khoa học: Molecular characterization of Arabidopsis thaliana PUF proteins – binding specificity and target candidates doc

... proteins are a large family of RNA-bindingproteins found in all eukaryotes. These proteins reducethe expression of mRNA targets by binding in 3¢ UTRregulatory elements, thus controlling translation ... cis-acting elements usuallylocated in the 3¢ UTR. In recent years, and as a result of great efforts aiming to understand the mechanism of RNA control in animals, the function of a diversenumber of ... predict that various PUF of A. thalina maybe involved in many different processes in the plant.RNA-binding capacity of the APUM proteinspfam analysis of all putative APUM proteins showedthat the...
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Tài liệu Báo cáo khoa học: Molecular characterization and allergenic activity of Lyc e 2 (b-fructofuranosidase), a glycosylated allergen of tomato pdf

Tài liệu Báo cáo khoa học: Molecular characterization and allergenic activity of Lyc e 2 (b-fructofuranosidase), a glycosylated allergen of tomato pdf

... isoform.Cloning of the cDNA of two isoforms of tomatob-fructofuranosidase and recombinant expression in E. coliFor protein expression in E. coli, only the cDNA coding forthe mature proteins ... participating in an IgE-bindingepitope. The N-terminal sequencing of a 52-kDa glyco-protein of tomato extract gave hints for the existence of b-fructofuranosidase as a relevant allergen in tomato[15,16]. ... was calculated as percent of thetotal amount of histamine determined after lysis of thebasophils by twofold freezing and thawing of the cells. Ahistamine release of more than 10% was considered...
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Tài liệu Báo cáo khoa học:

Tài liệu Báo cáo khoa học: "From HOPE en I''''ESPERANCE On the Role of Computational Neurolinguistics in Cross-Language Studies " pptx

... but are often generic and mark masculine, feminine, or plural (Gross, 1977; Goffic and McBride, 1975). And furthermore, these same articles often are not translated into meaning preserving sentences ... The study includes off-line picture naming which forces use of articles in all of the above contexts, as well as on-line production of these morphemes in an attempt to determine in which way ... behavior are introduced by including neurally motivated pro- cessing control as the focus of model definition and by including behaviorially defined con- straints, both normal and pathological....
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Tài liệu Báo cáo khoa học: Molecular cloning and characterization of soybean protein disulfide isomerase family proteins with nonclassic active center motifs pdf

Tài liệu Báo cáo khoa học: Molecular cloning and characterization of soybean protein disulfide isomerase family proteins with nonclassic active center motifs pdf

... processing), and GmPDIL-1 and GmPDIL-2 associate with proglycinin and b-con-glycinin in the ER, suggesting that they may playimportant roles in folding and in formation and rearrangement of disulfide ... synthesis levels of both b-conglycinin and glycinin,as pro-b-conglycinin and proglycinin are transient pro-tein forms that are present in the ER prior to process-ing in the protein storage vacuoles. ... proglycinin and b-conglycinin, and other ER molecular chaperonessuch as calnexin, calreticulin, BiP and PDI familyproteins, in vivo was not detected (data not shown),suggesting that GmPDIL-3a and...
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Tài liệu Báo cáo khoa học: Molecular modeling and functional characterization of the monomeric primase–polymerase domain from the Sulfolobus solfataricus plasmid pIT3 doc

Tài liệu Báo cáo khoa học: Molecular modeling and functional characterization of the monomeric primase–polymerase domain from the Sulfolobus solfataricus plasmid pIT3 doc

... and 80 °C between 190 and 260 nm with a step increase of 0.2 nm, and a bandwidth of 1 nm. Thermal stability of Rep245 was measured by incu-bating the protein at 60, 70 and 80 °C for 30 min and ... connects the a5 and a6 helices in thepRN1 prim–pol protein. Because of a two-residue gap in the corresponding region of the Rep245 sequence,this finding had not been predicted in phd and profsecondary ... activity of Rep245 and Rep516 proteins. (A) RNAprimer synthesis. Reaction mixtures, containing M13 single-stranded circular DNA, NTPs including [32P]ATP[aP], and Rep245(or Rep516), were incubated...
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Tài liệu Báo cáo khoa học: Molecular and biochemical characterization ofD-phosphoglycerate dehydrogenase fromEntamoeba histolytica A unique enteric protozoan parasite that possesses both phosphorylated and nonphosphorylated serine metabolic pathways docx

Tài liệu Báo cáo khoa học: Molecular and biochemical characterization ofD-phosphoglycerate dehydrogenase fromEntamoeba histolytica A unique enteric protozoan parasite that possesses both phosphorylated and nonphosphorylated serine metabolic pathways docx

... its role in the synthesis of L-cysteine and L-glycine and also in the formation of L-methionine by the interconversion of L-cysteine viaL-cystathionine,L-serineisamajorprecursorofphosphat-idyl-L-serine, ... of the nucleotidebinding domain of one of the adjacent subunits. Sitedirected mutagenesis of Trp139 to Gly resulted in thedissociation of the tetramer to a pair of dimers and in theloss of ... atthe nucleotide binding domains and the regulatorydomain, indicating the importance of these domains forthe tetramerization of the enzyme [18]. It was shown thatserine binding induces a conformational...
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Tài liệu Báo cáo khoa học: Molecular and functional characterization of adenylate kinase 2 gene from Leishmania donovani pdf

Tài liệu Báo cáo khoa học: Molecular and functional characterization of adenylate kinase 2 gene from Leishmania donovani pdf

... presence of three distinct domains: the rigidCORE-domain and two smaller peripheral domains ormobile parts, the NMP-binding site and the LID-domain[4]. The NMP-binding site has many intermolecularcontacts ... wereobtained by simple rotation of the same model. The flexible LIDdomain appears at top of the three figures, NMP-binding site and ATPbinding domain are displayed, NH2- and COOH-termini are alsoindicated.Ó ... Within the AMP bindingdomain, some amino acid residues are well conserved(L59, F87, R89) while two others, a leucine and a valine,are replaced by V35 and I60, respectively. A Mg2+binding...
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Tài liệu Báo cáo khoa học: Molecular basis of glyphosate resistance – different approaches through protein engineering doc

Tài liệu Báo cáo khoa học: Molecular basis of glyphosate resistance – different approaches through protein engineering doc

... accompanied by improved bind-ing to longer ligands (up to five atoms in the mainchain) and retained binding to shorter ligands [55]. Of the 21 changes in the evolution of R7 fromnative GLYAT ... thermo-stability and protein expression in E. coli determined for wild-type GO and variants of GO obtained by site-saturation mutagenesis of thepositions identified by docking analysis or by introducing multiple ... some of the key findings that have led to our current under-standing of the molecular mode of action of glypho-sate and the molecular basis for glyphosate resistance.Structure and function of...
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Tài liệu Báo cáo khoa học: Molecular defect of isovaleryl-CoA dehydrogenase in the skunk mutant of silkworm, Bombyx mori ppt

Tài liệu Báo cáo khoa học: Molecular defect of isovaleryl-CoA dehydrogenase in the skunk mutant of silkworm, Bombyx mori ppt

... leucine degradation and thesku mutant accumulates branched-chain amino acids in haemolymph, thismutant may be useful in the investigation of unique branched-chain aminoacid catabolism in insects.AbbreviationsACAD, ... the same molecular size and expression levels(Fig. 2A). Determination of full-length cDNAsequences using the RACE method in both strainsrevealed the presence of a single point mutation in 1337 ... investigatethe expression profile of the BmIVD gene. For bothwild-type and sku strain, a single band of 1.35 kb, cor-responding to the predicted molecular size of BmIVDmRNA, was detected in...
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