... o-NAC. The actual binding site of serotonin cannot be inferredfrom these results. Although serotonin can bind to the active site binding locus (W82), binding to PAS cannotbe ruled out. If serotonin ... matching the observationsobtained (Table 1). The binding of tyramine to D70G, in the presence of o-NPA, is weaker (9.1-fold) than binding to the wild-type enzyme, and induces an activating effect ... kinetic analysis of these inhibitingor activating effects was either incomplete [22,36–39]or debatable [33,42]. In particular, reported resultswere interpreted in terms of an AAA site distinct...