... Shiraki, School of Materials Science,Japan Advanced Institute of Science and Technology, 1-1 Asahidai,Tatsunokuchi, Ishikawa, 923-1292, Japan.E-mail: kshiraki@jaist.ac.jpAbbreviations:API,Achromobacter ... programINSIGHT II/DISCOVER(Accelrys Inc., San Diego, CA, USA) was used for energyminimization. The solvent accessible surface areas (ASA) of individual residues in the API variants were calculated ... mutants, the side-chain at position 169 remainedparallel with the side-chain of His210. On the other hand, a small side-chain at position 169, typically W169V and W16 9A, deviates from the original...