Tài liệu Báo cáo khoa học: ATPase activity of magnesium chelatase subunit I is required to maintain subunit D in vivo ppt

... Mg2+ is required to performthe ATP hydrolysis. In addition, kinetic analysis had shownbinding of Mg2+ to this subunit [21]. An integrin I domain,suggested to exist in the C-terminus of the ... BchI-BchD structure the ATPase activity of BchI is blocked. A conformational transition upon binding to BchH may bring the integrin I domain of BchD into contactwith the integrin-binding motif ... protein. In addition, the binding of BchDFig. 4. Coomassie-stained SDS/polyacrylamide gels. The ability of wild-type BchI and BchI with modiﬁcations D2 07N, L111F and R289K to bindHis-tagged BchD was...

Tài liệu Báo cáo khoa học: Autolytic activity of human calpain 7 is enhanced by ESCRT-III-related protein IST1 through MIT–MIM interaction pptx

... interactions by in vitro and in vivo bindingexperiments, using puriﬁed recombinant proteins andcultured mammalian cells expressing epitope-taggedproteins. We also investigated the effect of this interac-tion ... three-helix bundles. ESCRT-IIIproteins commonly contain conserved amino acidsequences for MIT binding, termed MIT-interactingmotif (MIMs), in their C-termini. Two types of MIMhave been identiﬁed: ... are important for binding to the VPS4 MIT domain are indicated byopen triangles. Wild-type (WT) as well asdeletion and amino acid substituted mutants of calpain 7 and IST1 used in this study...

Tài liệu Báo cáo khoa học: High activity of human butyrylcholinesterase at low pH in the presence of excess butyrylthiocholine pptx

... pKavalues of 6.0–6.6, suggestingthat the catalytic histidine is protonated at pH 5. Theexistence of activity when the catalytic histidine is protonatedindicates that the catalytic-triad mechanism of ... elimination of the bkcat activity required proto-nation of two amino acids. This biphasic titration accen-tuates the fact that the intermediate, singly protonatedspecies is active. That is ... of the protonated form of the catalytic histidine is inconsistent with the generally accepted mechanism forhydrolysis by cholinesterases [18,19]. This mechanismutilizes the catalytic histidine...

Tài liệu Báo cáo khoa học: Limited suppression of the interferon-b production by hepatitis C virus serine protease in cultured human hepatocytes pptx

... after virus infection. TRIF (adaptor pro-tein of Toll-like receptor 3)-mediated and Cardif (adaptor protein of RIG -I or MDA5)-mediated signaling pathways contribute rapid induction of interferon-b ... After dsRNA is recognized, the cytoplasmic domain of TLR3 recruitsTIR-domain-containing adaptor inducing IFN-b(TRIF) through a myeloid differentiation factor 88(MyD88)-independent pathway (TRIF-mediated ... pathwaysrapidly induce IFN-b through the phosphorylation of multiple cellular factors, including IFN regulatory fac-tor-3 (IRF-3) and kinases, including the Tank-bindingkinase 1 (TBK-1) and inhibitor...

Tài liệu Báo cáo khoa học: Mitochondrial targeting of intact CYP2B1 and CYP2E1 and N-terminal truncated CYP1A1 proteins in Saccharomyces cerevisiae ) role of protein kinase A in the mitochondrial targeting of CYP2E1 pdf

... containing phenylmethanesulfonyl ﬂuo-ride.Digitonin fractionationIsolated mitochondria (100 lg) were resuspended in SEMbuffer containing indicated concentrations of digitonin(0.05% to 0.4%, ... with depleted FDXR resulted in decreased activity (Fig. 9B) that was restored byadding bovine FDX + FDXR (Fig. 9B). Additionally,the enzyme activity was inhibited by antibody to human FDX (Fig. ... activity and mitochondrial CYP2E1 exhibitshigh NDMA -d activity. The activities of both micro-somal and mitochondrial enzymes were dependent onaddition of NADPH and inhibited by SKF-525. In summary,...

Tài liệu Báo cáo khoa học: The RNA recognition motif, a plastic RNA-binding platform to regulate post-transcriptional gene expression ppt

... inhibition element; PTB, polypyrimidine tract binding protein; RBD, RNA-binding domain;RNP, ribonucleoprotein; RRM, RNA recognition motif; SR, serine/arginine rich proteins; TLS, translocated in liposarcoma; ... nuclearribonucleoprotein; HuD, Hu protein D; LRR, leucine rich repeat; MIF4G, middle domain of the translation initiation factor 4 G; PABP,polyadenylate binding protein; PIE, polyadenylation inhibition ... protein cofactors can play an importantrole in RNA-binding speciﬁcity. This review also rai-ses many questions concerning this domain. First,concerning RNA binding, analysis of the differentstructures...

Tài liệu Báo cáo khoa học: The role of the SEA (sea urchin sperm protein, enterokinase and agrin) module in cleavage of membrane-tethered mucins pdf

... Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, NE, USASEA (sea urchin sperm protein, enterokinase andagrin) modules, minimally comprised of 120 aminoacids of ... SIVV motif alone as the ratio of cleaved to uncleaved protein is greater (Fig. 4B ,D) ,however, it is still highly inefﬁcient in comparison to MUC1FDTR (Fig. 4B ,D) .DiscussionThe function of the ... after translation in vivo and in vitro at the Gly-Ser peptide bond of the LSKG ⁄SIVVV motif [10], though additional amino acidsC-terminal to this site are also required for efﬁcientcleavage...

Tài liệu Báo cáo khoa học: Allosteric modulation of myristate and Mn(III)heme binding to human serum albumin Optical and NMR spectroscopy characterization pptx

... Allosteric and competition equilibria involving Mn(III)heme and myristate binding to HSA. Binding sites are indicated with thenotation (FA1)P(FAx)–(FAS), where FA1 is the heme binding site, acting ... Dipartimento di Biologia Strutturale e Funzionale, and Centro di Neuroscienze, Universita`dell’Insubria, Busto Arsizio (VA), Italy2 Dipartimento di Biologia, and Laboratorio Interdisciplinare di ... are depressed by myri-state, indicating that myristate afﬁnity for FA1 is higher than that of Mn(III)heme in the absence of myristate, and Mn(III)heme binding to FA1 is pre-cluded by competition...

Tài liệu Báo cáo khoa học: Crystal structure of thiamindiphosphate-dependent indolepyruvate decarboxylase from Enterobacter cloacae, an enzyme involved in the biosynthesis of the plant hormone indole-3-acetic acid doc

... tetramer interface.Residues lining the active site cavity are underlined. Residues binding ThDP are highlighted with a blue background, and those involved in substratebinding by yellow. Amino acids ... domains. The interface contains 10hydrogen bonds in IPDC. The dimer–dimer interface in IPDC is smaller than the corresponding interface in ZmPDC (4400 A˚2). It is signiﬁcantly larger than in ... and modelling [38] haveprovided considerable insights into the role of these residues in PDC. As all amino acids, which were suggested to participate in catalytic steps of PDC are conserved in...

Tài liệu Báo cáo khoa học: Structural effects of a dimer interface mutation on catalytic activity of triosephosphate isomerase The role of conserved residues and complementary mutations pptx

... listed in Table 1. In addition to the desired mutation, these primersalso contained restriction sites, incorporated by silent muta-genesis, in order to aid selection of recombinants. The sitesincorporated ... product (746 bp)containing the desired mutation was puriﬁed using a genecleaning kit (Qiagen, Qiagen India, Genetix Biotech Asia,New Delhi, India), digested with enzymes NcoI and BamHI,and ... Press, Cold Spring Harbor, NY.53 Bradford MM (1976) A rapid and sensitive methodfor the quantitation of microgram quantities of proteinutilizing the principle of protein–dye binding. AnalBiochem...

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