... endonucleases, T4 DNA ligase, E. coliDNA polymerase I Klenow fragment, and Taq DNA poly-merase were purchased from Fermentas (Hanover, MD,USA). Preparation of plasmids, and purification of PCRproducts ... organic phase bythree-fold extraction with 50% NaCl ⁄ chloroform (1 : 1v ⁄ v). A new volume of chloroform was used for each of the three extractions. Finally, the organic phase wasre-extracted ... at37 °C for 18 h. Sensitivity was assessed by measuring thediameter of the zone of growth inhibition in two axes, andthe mean value was calculated.Bactericidal assay with normal human serumComplement-sufficient...