... parameters of chitinase A with various substrates.The hydrolysis of chitooligosaccharides and colloidal chitin at sub-strate concentrations of 0–2 mM was carried out with 75 ng of native chitinase ... Research Scholar of the ThailandResearch Fund. We would like to thank Prof. Steve C.Fry, Institute of Cell and Molecular Biology, Univer-sity of Edinburgh, Edinburgh, UK, and Dr AlbertSchulte, ... 70 mgÆL)1 of purifiedprotein. SDS ⁄ PAGE analysis followed by staining with Coomassie blue showed single bands for thewild-type and mutants D392N and E315M, migrating with an Mr of 63 000...