... 53, 10 81 10 84 . 15 Tsuruoka N, Nakayama T, Ashida M, Hemmi H,Nakao M, Minakata H, Oyama H, Oda K & Nishino T(2003) Collagenolytic serine-carboxyl proteinase from Alicyclobacillus sendaiensis ... enhance-ment of the 57-kDa band with a concomitant diminu-tion of the 38- kDa and 19 -kDa bands, as analyzed bySDS ⁄ PAGE (Fig. 3A) . The 38- kDa and 19 -kDa bandsdid not disappear completely after ... well as Asn164 of the E78H ⁄ D164Nmutant precursor. Thick lines indicate the polypeptide chain of the 38- kDa mature form of the mutant. C 17 2p a ,C 17 3p a ,andC364 a denote a car-bons of His172p,...