Tài liệu Báo cáo khoa học: Characterization of the interaction between the plasma membrane H+-ATPase of Arabidopsis thaliana and a novel interactor (PPI1) doc

Tài liệu Báo cáo khoa học: Characterization of 1H NMR detectable mobile lipids in cells from human adenocarcinomas doc

... from human adenocarcinomasAnna Maria Luciani1, Sveva Grande1, Alessandra Palma1, Antonella Rosi1, Claudio Giovannini2,Orazio Sapora3, Vincenza Viti1 and Laura Guidoni11 Dipartimento ... irradiated: black) and A ⁄ (Lys + Ala) (controls: dotted white;irradiated: dotted black) as obtained from 1D and 2D COSY spectra of HeLa and MCF-7cell samples. Data are the mean ± SDvalues of ... arachidonic acid chains, and at 0.93–2.04 p.p.m., attributed to linolenic acid chainson the basis of a comparison with lipid extracts and from the data available in the literature, are alsoclearly...

Tài liệu Báo cáo khoa học: Characterization of chitinase-like proteins (Cg-Clp1 and Cg-Clp2) involved in immune defence of the mollusc Crassostrea gigas docx

... (5¢-GCATAGCGATGTGGACGA-3¢) and QaCgClp2 (5¢-GAGGACCGAGACCGTGAA-3¢). The abbreviations ‘Qs’ and ‘Qa’ refer, respectively, to sense and antisense primers. Accurate ampliﬁcation of the targetamplicon was checked by obtaining ... and is characterizedby the degeneration of larval tissues, such as the velum and the foot, and the remodelling of larval tissues toproduce adult tissues (i.e. the development of the gills and ... sequencingchemistry.Real-time quantitative PCRQuantitative RT-PCR analysis was performed using the iCycler apparatus (Bio-Rad, Hercules, CA, USA). TotalRNA was isolated from oocytes, embryos, larvae and adulttissues...

Tài liệu Báo cáo khoa học: Characterization of human deoxyribonuclease I gene (DNASE1) promoters reveals the utilization of two transcription-starting exons and the involvement of Sp1 in its transcriptional regulation ppt

... (Promega),followed by assay of DNase I and b-galactosidase activities,according to a previously described method [32].Preparation of nuclear extracts and EMSA The nuclear extract and probe were prepared as reportedpreviously ... cells and a primer set of the forward pri-mer DN-5R and the reverse primer DN+854X (sequences5¢-CGGAATTCTCAGGATGAGGGGCATGAAG-3¢ and 5¢-CGCTCGAGGCTGCTCACTTCAGCATCAC-3¢, res-pectively) and directionally ... Kominato Y, Yamamoto F & Takizawa H(2002) Characterization of the human ABO gene pro-moter in erythroid cell lineage. Vox Sang 82, 39–46.34 Yasuda T, Awazu S, Sato W, Iida R, Tanaka Y &Kishi...

Tài liệu Báo cáo khoa học: Characterization of the interaction between the plasma membrane H+-ATPase of Arabidopsis thaliana and a novel interactor (PPI1) doc

... Characterization of the interaction between the plasma membrane H+-ATPase of Arabidopsis thaliana and a novel interactor (PPI1) Corrado Viotti, Laura Luoni, Piero Morandini and Maria Ida ... plasma membrane receptor and the activation of the plasma membrane H+-ATPase. IV. Fusicoccin induces the association between the plasma membrane H+-ATPase and the fusicoccin receptor. Plant ... novel interactor of the C-ter-minus of Arabidopsis thaliana plasma membrane H+-ATPase (EC 3.6.3.6)(Morandini P, Valera M, Albumi C, Bonza MC, Giacometti S, Ravera G,Murgia I, Soave C &...

Tài liệu Báo cáo khoa học: Characterization of a recombinantly expressed proteinase K-like enzyme from a psychrotrophic Serratia sp. ppt

... 5¢-CTTTAACTTGTTGGGCACTGGCATTG-3¢; NP6, 5¢-TTGATCGATTCTGTCTATGCCCCA-3¢ along with the adaptor primers: AP1 (5¢-GTAATACGACTCACTATAGGGC-3¢) and AP2 (5¢-ACTATAGGGCACGCGTGGT-3¢).Nested PCR was carried ... endo- and exo- peptidases are widespread innature and found in viruses, archaea, bacteria and euk-aryotes. The biological importance of peptidases areclearly indicated by the fact that 2% of all ... PCR was performed in 50 lL containing 1 ng of genomic DNA as template, 0.2 mm dATP, dCTP, dGTP and dTTP, 0.2 lm of upstream primer (OP17: 5¢-GAAAAACCATGGTGAATGAATACCAAGCGACT-3¢ ) and downstream...

Tài liệu Báo cáo khóa học: Characterization of the products of the genes SNO1 and SNZ1 involved in pyridoxine synthesis in Saccharomyces cerevisiae pptx

... 5¢-ATACCATGGACAAAACCCACAGTACAATGP1OH 5¢-CATATGCACAAAACCCACAGTACP2O 5¢-TATGGATCCTTAATTAGAAACAAACTGTCTGATAAACP2OH 5¢-CTCGAGATTAGAAACAAACTGTCTGATAAACCP1Z 5¢-ATACCATGGCTGGAGAAGACTTTAAGATCP1ZH ... 5¢-CATATGACTGGAGACTTTAAGATCP2Z 5¢-TATGGATCCTCACCACCCAATTTCGGAAAGP2ZH 5¢-CTCGAGCCACCCAATTTCGGAAAGTTable 2. Over-expression plasmids for Sno1p and Snz1p prepared in thisstudy. Symbols O, Z and ... min, the absorbance of the supernatant was read at 363 nm. The absorbance of the reduced form of APAD (APADH) was linear over the 2–100 lMrange of glutamate with a molar extinctioncoefﬁcient of...

Tài liệu Báo cáo khoa học: Characterization of promoter 3 of the human thromboxane A2 receptor gene A functional AP-1 and octamer motif are required for basal promoter activity docx

... Aiba Y, Nakamura K, Namba T, HirataM, Mazda O, Katsura Y & Narumiya S (1993)Thromboxane A2 receptor is highly expressed in mouseimmature thymocytes and mediates DNA fragmentation and apoptosis. ... Ireland The prostanoid thromboxane (TX )A 2induces activa-tion and aggregation of platelets, constriction of vascu-lar (V) and bronchial smooth muscle (SM) and of renal mesangial cells [1–4], and ... Grazia U, Felli MP, Vacca A, Farina AR, Maroder M,Cappabianca L, Meco D, Farina M, Screpanti I, Frati Let al. (1994) Positive and negative regulation of the com-posite octamer motif of the...

Tài liệu Báo cáo khoa học: Characterization of electrogenic bromosulfophthalein transport in carnation petal microsomes and its inhibition by antibodies against bilitranslocase docx

... asso-ciated with the plasma membrane of epidermal cells.At this magniﬁcation, the vacuolar membrane and the plasma membrane could not be resolved, because the vacuole takes a large part of the ... Àðk A =k0Þ= A ð1Þwhere k A and k0are the inactivation rate constantseither in the presence or in the absence of variousconcentrations of a ligand A, k2 and k1are the rate constants of ... 1), plasma membrane (lane 2) and tonoplast (lane3) vesicles obtained from carnation petals, while lane 4was loaded with rat liver plasma membrane vesicles. Inall samples, antibodies A and...

Tài liệu Báo cáo khoa học: Characterization and mode of action of an exopolygalacturonase from the hyperthermophilic bacterium Thermotoga maritima doc

... tubingensis (CAA68128.1), Pgx2 Arabi-dopsis thaliana (AAF21195.1). The mode of action (endo or exo) and the amount of GalpA cleaved off, respectively, are annotated in paren-theses. A question mark indicates ... of pectin areclassiﬁed as members of family 28 of the glycosidehydrolases, including the endopolygalacturonases, exo-polygalacturonases and rhamnogalacturonases [3,4].Although a handful of ... bacterial exo-acting polygalacturonasescommonly generate digalacturonate, PelB was shownto liberate monogalacturonic acid as the ﬁrst and onlyproduct on PGA and oligoGalpA. On the basis of...

Tài liệu Báo cáo khoa học: Characterization of ICAM-4 binding to the I domains of the CD11a/CD18 and CD11b/CD18 leukocyte integrins pptx

... I domain of CD1 1a, and partially but signiﬁcantly inhibited the interaction between ICAM-4 L cells and CD1 1a I domain. The adhesion of all ICAM transfectants to the I domain of CD1 1a was almost ... domain GSTfusion proteins migrated as major bands of  50 kDa. AfterFactor X or thrombin cleavage and removal of GST the I domains appeared as single major bands of approximately24 kDa (CD1 1a) ... ICAM-4transfectants to the I domains of CD1 1a and CD11b and compared the results to the divalent cation requirements of ICAM-1 and ICAM-2 transfectants. We also analyzed the cation dependence of red cell adhesion...

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