... effect of Pi on the subtilisin digestion
indicates stabilization of the D-loop.
Monitoring the binding of cofilin to F -actin by
limited proteolysis
The effect of Pi on the binding of cofilin to F -actin
was ... to F -actin is pertinent to the regulation
of actin dynamics in the cell.
Results
Fluorescence measurements of the effect of Pi
on...
... HIF-1-related effect of hypoxia on the expression of
the critical hydroxylases for collagen fiber formation, this
study aimed to characterize the influence of hypoxia on the
gene expression of these hydroxylases ... hydroxylases. With the exception of PDI, which
is known to be induced by the UPR [41], however, none of
the other enzymes was relevantly induced by th...
... which
synthesizes components of the cuticle, may offer a useful
target for studies into the mechanism of the development
and molting process in the roundworms. Investigation into
the effects of fluoride ... musculature. The ecdysis of
an old cuticle and deposition of the components of a new
cuticle that are synthesized in the hypodermis and are
secreted across the...
... Furthermore, another
three reporter vectors containing mutations of the
miR-23a binding site were used in the fluorescent
reporter assay. The reporter vector containing a muta-
tion of the first binding ... a
potential regulatory pathway in which the upregulated
expression of miR-23a causes the downregulation of
IL6R by binding to its first conserved binding site,
w...
... may enter the citric acid
cycle completing the catabolism of the pyrrolidine moiety of nicotine.
Expression of the genes of these enzymes was dependent on the presence of
nicotine in the growth ... University of Freiburg, Germany
One of the major health risks continues to be the smok-
ing of tobacco. Nicotine, in itself highly toxic, when
inhaled with the t...
... the
function of the seven supernumerary subunits. To gain fur-
ther insight into the function of the supernumerary subunits
in the assembly process, we have examined the subunit
composition of ... supernumerary
subunits. Deletion of any one of the genes encoding cyto-
chrome b, subunit 7 or subunit 8 caused the loss of the other
two subunits. This is consistent...
... man [8,9]. In the verteb-
rate lens, the b and c crystallins together account for
the majority of the soluble proteins (the other major
family being the a-crystallins, members of the small
heat-shock ... different regions
of the eye. The expression of cN in the central regions
of the lens, fiber cells that were laid down before birth,
is consistent with the fam...
... screening procedures to take
into account the conditions found in the cell cytoplasm
and to allow the selection of binders of sufficient affin-
ity for interacting with their target in the crowded
cellular ... the
amplification of the 3¢ end of the b-gal gene. The presence
of similar amounts of genomic DNA in the reactions was
controlled by concomitant addition...
... that regulates the dur-
ation of receptor activation, is mediated by the protea-
some [7]. Also, it is becoming increasingly clear that
the degradation of members of the family of regulators
of ... degradation could be due
to the inability of the transfected receptors to activate
the G proteins. To investigate this, the effect of ligand-
activation on ino...
... primarily
due to the obligatory treatment of the anemia with blood
transfusions. The contribution of plasma NTBI to the
toxicity associated with Fe overload in these disorders is
uncertain, as is the form ... 1693
Effect of chelators and Fe status on subcellular distribu-
tion of NTBI in hepatocytes. The effect of the chelators on
the intracellular distribution o...