Tài liệu Báo cáo khoa học: Crystal structures of a bacterial 6-phosphogluconate dehydrogenase reveal aspects of speciﬁcity, mechanism and mode of inhibition by analogues of high-energy reaction intermediates pdf

Tài liệu Báo cáo khoa học: Crystal structures of the regulatory subunit of Thr-sensitive aspartate kinase fromThermus thermophilus pdf

... DataBank (accession numbers 2dt9 and 2zho).Determination of quaternary structureThe subunit organization of TtAKb was analyzed by analyt-ical ultracentrifugation and gel ﬁltration chromatography.Fig. ... biosyntheticpathway catalyzed by a monofunctional aspartokinase.Acta Crystallogr F 62, 962–966.20 Yoshida A, Tomita T, Kurihara T, Fushinobu S,Kuzuyama T & Nishiyama M (2007) Structural insightinto ... differential scanning calorimetry; EcAKIII, aspartate kinase IIIfrom Escherichia coli; MAD, multiwavelength anomalous diffraction; MjAK, aspartate kinase from Methanococcus jannaschii; TtAK, aspartatekinase...

Tài liệu Báo cáo khoa học: Crystal structures of a bacterial 6-phosphogluconate dehydrogenase reveal aspects of speciﬁcity, mechanism and mode of inhibition by analogues of high-energy reaction intermediates pdf

... The Authors Journal compilation ª 2006 FEBS Crystal structures of a bacterial 6-phosphogluconate dehydrogenase reveal aspects of speciﬁcity, mechanism and mode of inhibition by analogues of high-energy reaction ... suite: programs for protein crystallo-graphy.Acta Crystallogr D Biol Crystallogr 50, 760–763.28 Navaza J (1994) Amore – an automated package formolecular replacement. Acta Crystallogr A 50, 157–163.29 ... 25% of the accessiblesurface area of a monomer contributes to LlPDHdimer formation. Whereas LlPDH and OaPDH have a buried surface area of  5500 A ˚2, TbPDH has a largerinterface surface area...

Tài liệu Báo cáo khoa học: Crystal structure of an ascomycete fungal laccase from Thielavia arenaria – common structural features of asco-laccases ppt

... ascomycete fungal laccase fromThielavia arenaria – common structural features of asco-laccasesJuha P. Kallio1, Chiara Gasparetti2, Martina Andberg2, Harry Boer2, Anu Koivula2, Kristiina Kruus2,Juha ... ElectronCorporation, Waltham, MA, USA). The reactions werestarted by addition of substrate, and the rate of substrateoxidation was measured by monitoring the change in absor-bance over 5 min. All of ... copper-containing enzymes used in various applications, suchas textile bleaching. Several crystal structures of laccases from fungi and bacteria are available, but ascomycete types of fungal laccases...

Tài liệu Báo cáo khoa học: Crystal structure of importin-a bound to a peptide bearing the nuclear localisation signal from chloride intracellular channel protein 4 ppt

... atoms) and rjis the standard deviation of Bfactors. The normalized B factors have a zero mean and unitvariance. All atoms that satisfy Bz‡ 4 are treated as outliers and discarded. After ... (1999) Autoinhibition by an internal nuclearlocalization signal revealed by the crystal structure of mammalian importin alpha. Nat Struct Biol 6, 388–397.12 Hodel MR, Corbett AH & Hodel AE ... importin -a nuclear import pathway, provided that CLIC4 can undergo a conforma-tional rearrangement that exposes the NLS in an extended conformation.DatabaseStructural data are available in...

Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf

... structure and catalysis. Biochemistry 48, 3417–3424.31 Nakamura T, Matsumura H, Inoue T, Kai Y, UegakiK, Hagihara Y, Ataka M & Ishikawa K (2005)Crystallization and preliminary X-ray diffractionanalysis ... Suzuki5 and Yasushi Kawata3,41 National Institute of Advanced Industrial Science and Technology, Ikeda, Osaka, Japan2 Department of Food Science and Nutrition, Faculty of Human Life and Science, ... hyper-thermophilic crenarchaeon, Aeropyrumpernix K1. DNA Res 6, 83–101.11 Nakamura T, Yamamoto T, Inoue T, Matsumura H,Kobayashi A, Hagihara Y, Uegaki K, Ataka M, Kai Y& Ishikawa K (2006) Crystal structure...

Tài liệu Báo cáo khoa học: Crystal structure of Klebsiella sp. ASR1 phytase suggests substrate binding to a preformed active site that meets the requirements of a plant rhizosphere enzyme doc

... RHGXRXP and HD, and hydrolyze metal-free phytate with pH optima in theacidic range. They consist of two domains, a large a ⁄ bdomain and a small a domain with the catalytic site atthe interface of ... glucose-1-phosphatase and human prostatic-acid phosphatase. The polypeptidechain is organized into an a and an a ⁄ b domain, and the active site islocated in a positively charged cleft between the domains. ... substrate-freeAppA the C a atoms are 2.41 A ˚apart, whereas for thesubstrate-free PhyK and the substrate-loaded AppAthe averaged distance is only 1.87 A ˚.Distinct conformational changes...

Tài liệu Báo cáo khoa học: Crystal structure of the catalytic domain of DESC1, a new member of the type II transmembrane serine proteinase family pptx

... reactive loop sequences areVal-Ser-Ala-ArgflMet-Ala-Pro and Phe-Thr-Phe-Argfl-Ser-Ala-Arg for PAI-1 and PCI, respectively [6]. By contrast, the reactive site loops of a 1-antichymotrypsin and ... that theSEA domain functions by orienting the active site cleft of DESC1 toward plasma and ⁄ or extracellular spaces and away from the cell surface and ⁄ or the extracellularmatrix. The SEA ... supernatantwas clarified and collected, and DESC1 was purified by using affinity chromatography on a benzamidine columnfollowed by anion exchange chromatography on a Q-Seph-arose column (Amersham...

Tài liệu Báo cáo khoa học: Crystal structure of the BcZBP, a zinc-binding protein from Bacillus cereus doc

... Ivanova N, Sorokin A, Anderson I, Galleron N,Candelon B, Kapatral V, Bhattacharyya A, Reznik G,Mikhailova N, Lapidus A et al. (2003) Genomesequence of Bacillus cereus and comparative analysiswith ... partfolds into a structure consisting of two hydrogen-bon-ded antiparallel b-strands (b6–b7), an a- helical hairpin(helices a5 , a6 ) and a C-terminal strand (b8). Helix a5 and strand b7 partially cover ... ﬁrst two zinc ligands being separated by a shortsegment of 1–3 residues and the last two ligands beingseparated by a segment of variable length and with noparticular amino acid preferences...

Tài liệu Báo cáo khoa học: Isochore structures in the chicken genome ppt

... Andreozzi L, Federico C, Motta S, Saccone S, Sazano-va AL, Sazanov AA, Smirnov AF, Galkina SA, LukinaNA, Rodionov AV et al. (2001) Compositional mapping of chicken chromosomes and identiﬁcation ... T, Yamagata T, Fukagawa T, Sugaya K, Ando A, Inoko H, Gojobori T, Fujiyama A, Okumura K &Ikemura T (1997) Precise switching of DNA replicationtiming in the GC content transition area in ... evolutionary gap between mam-mals and other vertebrates and serves as a mainlaboratory model for the $ 9600 extant avian species.The chicken also represents the ﬁrst agricultural ani-mal to have...

Tài liệu Báo cáo khoa học: ˚ The 1.8 A crystal structure of a proteinase K-like enzyme from a psychrotroph Serratia species docx

... The ResearchCouncil of Norway and Biotec Pharmacon ASA.References1 Rao MB, Tanksale AM, Ghatge MS & Deshpande VV(1998) Molecular and biotechnological aspects of micro-bial proteases. Microbiol ... N-terminal pro domain is autocatalytically cleavedoff when the enzyme has obtained its active conforma-tion, and the two C-terminal domains are cleaved off by heat treatment at 50 °C. An Ala-Pro-Thr ... amideoxygen atom of Gln15 and the carbonyl oxygen atoms of Asp11 and Asn23 (Fig. 3) in an arrangement similarto what is observed in VPRK. Both PRK and VPRKhave calcium bound at Ca3. SPRK also...

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