... charged amino acid sidechains, R397 and R120, are also involved in fosfomycinbinding, and in view of the multitude of interactions, it wasassumed that deletion of a single interaction would not ... leads to a more detailed understanding of the role of K22 and R120 with respect to fosfomycin binding as wellas the ligand-induced conformational switch.Experimental proceduresChemicals and ... Temperature dependence of DH (circles, solid lines), DG(squares, dotted line) and –TDS (triangles, dashed lines) for UDPNAGbinding to the K22VR120K (A), K22V (B) and R120K mutant proteins(C). Thermodynamic...