Tài liệu Báo cáo khoa học: Functional studies of active-site mutants from Drosophila melanogaster deoxyribonucleoside kinase Investigations of the putative catalytic glutamate–arginine pair and of residues responsible for substrate speciﬁcity docx

... from Drosophila melanogaster deoxyribonucleoside kinase Investigations of the putative catalytic glutamate–arginine pair and of residues responsible for substrate speciﬁcity Louise Egeblad-Welin1,2,*, ... (BVDU). Mutants of catalytic residues: E52 and R105It is evident from the kinetic results that mutation of E52 changes only the catalytic rate. For the E52Dmutant the Kmvalue was approximately the ... 2007)doi:10.1111/j.1742-4658.2007.05701.x The catalytic reaction mechanism and binding of substrates was investi-gated for the multisubstrate Drosophila melanogaster deoxyribonucleoside kinase. Mutation of E52 to D, Q and H plus...

Tài liệu Báo cáo khoa học: Comparative studies on the functional roles of N- and C-terminal regions of molluskan and vertebrate troponin-I pdf

... In the present study, we compared the functional roles of the N- and C-terminal regions of molluskan and vertebrate TnI and revealed for the ﬁrst time that (a) the alternative binding of the ... Lanes a and d, in the absence of both TnC and Ca2+; lanes b and e, in the presence of TnC and the absence of Ca2+; lanes c and f, in the presence of bothTnC and Ca2+. Ac, actin; Tm, tropomyosin; ... ATnI130)252, and ATnI232)292did not migrate into the gels, while free ATnI-52K and ATnI1)128exhibited a band near the origin and at the middle of the gel, respectively. The bands corresponding to the...

Tài liệu Báo cáo khoa học: Functional and structural analyses of N-acylsulfonamidelinked dinucleoside inhibitors of RNase A ppt

... probes are useful for both studying and controlling the functions of enzymes and other proteins. The most useful probes have high afﬁnity for their target, along with small size and resistance to ... 123,2937–2945.50 Burlingham BT (2002) Design and synthesis of chemicalprobes for the investigation of enzyme recognition of anionic substrates. PhD Thesis, Department of Chemistry, Indiana University, ... bonds with the enzyme, and O2¢ forms two additional hydrogen bondsin each of its conformations. Thus, in either observedconformation of N-acylsulfonamide 7, there are a total of four hydrogen...

Tài liệu Báo cáo khoa học: Functional characterization of an orphan cupin protein from Burkholderia xenovorans reveals a mononuclear nonheme Fe2+-dependent oxygenase that cleaves b-diketones ppt

... is assigned to the mixing of the 4p orbital with the 3d orbital of the metal cofactor.Two important pieces of information can be gleaned from the pre-edge peak. First, occurrence of this tran-sition ... with one of the initial models considered (ﬁt 1). See Table S1 for further details of EXAFS data analysis. N is the number of ligands, r is the distance to the central iron atom, and r2is the Debye–Waller ... ligand binding, and the role of storage proteins, the cupins constitute one of the mostdiverse superfamilies of proteins known. They havebeen described from all three domains of life [1,2], and usually...

Tài liệu Báo cáo khoa học: Comparative studies of endonuclease I from cold-adapted Vibrio salmonicida and mesophilic Vibrio cholerae docx

... that the rate of irreversible unfolding for VsEndA is higher than for Vc EndA, with a half-life of % 13 and 33 min,respectively. Substrate specificity analysisAn analysis of the substrate specificity ... specificity for DNA of the enzymes shows that they both cleave plasmid DNA,dsDNA and ssDNA as shown in Fig. 8.To test the RNA specificity of the enzymes, we used the RNaseAlert assay and compared the ... toboth salinity and temperature. The Km of VsEndA ishigher than that of VcEndA; therefore, the more posit-ive surface of VsEndA does not seem to significantlyincrease the affinity for the negatively...

Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt

... deletion of 9 3 residues (DEC1:94–412) or more than 93 residues ( DEC1:119–412)abolished the suppression (Fig. 1A). On the other h and, deletion of up to 273 residues from the C-terminal region of ... binding to the E-box (lanes 6 and 16). On t he other hand, deletion of up to 273 residues from the C-terminal region of DEC1 (VP16-DEC1:4–232 and VP16-DEC1:4–139) did not diminish the b indingactivity ... together with the resultsshown in Figs 1–3, these ﬁndings indicate that the regionrequired for the suppressive activity of DEC1 is alsorequired for the interaction with BMAL1.Binding of DEC1...

Tài liệu Báo cáo khoa học: Functional hierarchy of plasminogen kringles 1 and 4 in ﬁbrinolysis and plasmin-induced cell detachment and apoptosis docx

... inhibit the LBS function of either K1[34] or K4 [35] and have used them to analyse, in functional isolation, the role of these LBS on plas-min(ogen) binding and activation. The effect of thesemAbs ... (inset) in the presence of varying concentrations of mAb A10.2 or mAb 34D3 (0–1 lM for cells and 0–0.25 lM for fibrin) and fixedconcentrations of Glu-Pg (200 nM for cells and 50 nM for fibrin) and CBS0065 ... nonspecificeffects of intact mAbs, including the reproduction of similar results with their Fab fragments and the absence of effect on plasminogen binding and activa-tion in the presence of the unrelated...

Tài liệu Báo cáo khoa học: Functional interaction between RNA helicase II⁄Gua and ribosomal protein L4 pptx

... component of 60S ribosome large subunit. The ATPase activity of Gua is important for this interaction and is also necessary for the func-tion of Gua in the production of both 18S and 28S rRNAs. ... The observed difference in the sensitivity of the two experiments (Fig. 2A,B)might be attributed to difference in the levels of expression of FLAG-Gua and FLAG-RPL4. The sig-nal intensity of ... comparing the signalintensities of the precipitates with those of the originalinputs (Fig. 2B). The ratio (input : IP) is approxi-mately 1 : 5 for wild-type, 1 : 1 for SAT mutant and 5 : 1 for DEVD...

Tài liệu Báo cáo khoa học: Functional dissection of the Schizosaccharomyces pombe Holliday junction resolvase Ydc2: in vivo role in mitochondrial DNA maintenance pptx

... crossover – and the unpairing of four base-pairs in the centre of the junctions [19]. Detailedunderstanding of the functional role of these interactionswould require the crystal structure of the Ydc2–junctioncomplex.In ... Binding of CCE1 results in the complete unpairing of the four base-pairs in the centre of the junctions with a lesser disruption of the next base-pairs[19], which is reminiscent of the structure of ... with the arms of the junction are needed for the correct positioning of the catalytic sites for cleavage, whichmay involve the steric ÔpinÕ ) the loop protruding betweenhelices 3 and 4 at the...

Tài liệu Báo cáo khoa học: Functional effects of deleting the coiled-coil motif in Escherichia coli elongation factor Ts pptx

... C of the core domain and contains the protruding antiparallel coiled-coil motif (helices 10 and 11, residues 187–203 and 208–226) responsible for the dimerization of EF-Ts in the crystal. In the ... the present results indicate that the maximal rate of the mutantEF-Ts in protein synthesis in vivo is  70%ofthatofthewild-type EF-Ts. The studies of the effect of the EF-Ts mutation understarvation ... between the levels and the productionpatterns of the (p)ppGpp in the two strains, either beforeor during the full stringent response (Fig. 4B). Growth rates in the presence of kirromycin The effect...

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