Tài liệu Báo cáo khoa học: It is all about resolution Meeting report based upon presentations at the 10th International Global BioMillennium 2006 symposium on molecular cell biology (Tbilisi, Georgia) docx

... ARTICLE It is all about resolution Meeting report based upon presentations at the 10th International Global BioMillennium 2006 symposium on molecular cell biology (Tbilisi, Georgia) Hermona Soreq1and ... methylations are epigeneticallymaintained via methyltransferases that associate withproteins that recognize the methylated epitope andpropagate the silent state. Together, these observationsKeywordsalternative ... functional features. The sym-metry relates the RNA backbone to nucleotideorientation, but shows no sequence homology. Thisdemonstrates the superiority of function oversequence conservation,...

Tài liệu Báo cáo khoa học: NirF is a periplasmic protein that binds d1 heme as part of its essential role in d1 heme biogenesis pdf

... and ﬁnally reduction ofnitrous oxide to nitrogen. In the denitriﬁcation path-way, nitrite reduction is the key step, as it is the pointof divergence from assimilatory nitrogen metabolism inwhich ... pantotrophus catalyses the one electron reduction of nitrite to nitric oxide using two heme cofactors. The site of nitrite reduction is the d1heme, which is synthesized under anaer-obic conditions by ... supplemented with 20 mm nitrate, it converted the entire available nitrate to nitrite within10 h of growth and lost its nitrite reductase activity, asshown by no consumption of nitrite to yield...

Tài liệu Báo cáo khoa học: Bacitracin is not a speciﬁc inhibitor of protein disulﬁde isomerase pptx

... Although there have been reportsthat there is protease contamination of some commerciallyavailable bacitracin preparations [31], we were loathe tofractionate the material to ensure that there ... aggregation and the chaperone activity of BiPIn addition to disulfide bond formation, PDI has beenshown to have chaperone-like activity [34]. As rhoda-nese contains no disulfide bonds, and is prone ... ofdisulfide bonds. The effects of bacitracin on this activitywere examined using the insulin precipitation assay. In the noncatalyzed assay, the disulfides of insulin arereduced by dithiothreitol,...

Tài liệu Báo cáo khoa học: "It Would Be Much Easier" doc

... learnt the V set and F3 mapping. At this point, the activity of the designer Is theoretically finished and it is the system itself which wlU generate, based on these definitions, the paradigmatic ... column with the In. flected forms of the thematic word. Each word form must obey the restrictions Imposed by the combination of the feature values displayed on the line which the tutor is writing ... (Tufis,1987a) incorporated by the lURES environ- ment for building natural language applications (Tufts, 1985). tioned motivations. With respect to the second one maybe it is worth saying that...

Tài liệu Báo cáo khoa học: Synchronization of Ca2+ oscillations: a coupled oscillator-based mechanism in smooth muscle ppt

... depo-larization. On the basis of experimental and theoreticalconsiderations, we now consider how Ca2+oscillationscan be synchronized across multiple cells in a syn-cytium.Synchronization of ... mechanism is a coupledoscillator -based synchronization of Ca2+oscillations across cells thatdrives membrane potential changes and causes coordinated contractions. The key elements of this mechanism ... activation ofIns(1,4,5)P3(Fig. 3), with the increased cytosolicIns(1,4,5)P3concentration causing generation of oscil-lations in cell 2. Importantly, although the frequencyof the oscillations...

Tài liệu Báo cáo khoa học: ¨ Induction of Kruppel-like factor 4 by high-density lipoproteins promotes the expression of scavenger receptor class B type I pptx

... the binding to putative KLF4 bindingelement on the promoter of SR-BI. All results indicate a potential functionof KLF4 in the pathogenesis of atherosclerosis through the regulationeffect on ... signalling, implicatingKLF4 as a regulator of key signalling pathways thatcontrol macrophage activation [3]. Furthermore, it hasalso been demonstrated that KLF4 is required for the expression ... abolished upon further point mutations of the corresponding KLF4binding site. The speciﬁcity of transcriptional activity ofKLF4 on SR-BI promoter was further conﬁrmed byanother transcription factor,...

Tài liệu Báo cáo khoa học: Cobalamin uptake and reactivation occurs through speciﬁc protein interactions in the methionine synthase–methionine synthase reductase complex docx

... 2and 4 (in the case of full-length MSR) reduced states bytitration with dithionite. The UV–visible spectra of the ﬂavoproteins were recorded with sequential addition ofdithionite. The various ... reaction volume was 0.8 mL. The reactions were initiated by the addition of homo-cysteine following 5 min of incubation at 37 °C of the enzyme with all other components. Following 10 min ofincubation ... activity on MSR concentration,illustrating the optimal concentration of MSR required for reductivemethylation (reactivation) of the cob(II)alamin form of hMS. hMSassays were performed using the...

Tài liệu Báo cáo khoa học: Molecular and cellular speciﬁcity of post-translational aminoacyl isomerization in the crustacean hyperglycaemic hormone family docx

... of the X organ–sinus gland system? Inother words, what is the cellular speciﬁcity of the isom-erization process? To answer this question, the distri-bution of VIH and CHH isomers within the ... from the cell body to the axon terminal in the sinus gland, as a result of late and progressiveisomerization of the Phe3 of the CHH during the migration of the secretion vesicles along the axonaltract ... VIH isomers in the lobster X organ, oneattractive simple hypothesis was that the mixed(double labelled) cells observed previously could con-stitute the synthesis site of the d-epimer of the...

Tài liệu Báo cáo khoa học: Substrate speciﬁcity and inhibition of brassinin hydrolases, detoxifying enzymes from the plant pathogens Leptosphaeria maculans and Alternaria brassicicola ppt

... degradation by pathogens could increase the concentrations of plant defences and decrease the needto apply fungicides at the onset of disease.Recently, it was shown that a group of isolates of the ... involved in the catalyticactivity of both enzymes. The overall results indicated that BHs have newsubstrate speciﬁcities with a new catalytic activity that can be designated asdithiocarbamate hydrolase. ... 7412–7428 ª 2009 The Authors Journal compilation ª 2009 FEBS at a position corresponding to a molecular mass of about 120 kDa. Thus, these data suggest that nativeBHAb is a dimer.Identiﬁcation and chemical...

Tài liệu Báo cáo khoa học: Mechanism of dihydroneopterin aldolase NMR, equilibrium and transient kinetic studies of the Staphylococcus aureus and Escherichia coli enzymes docx

... reaction. Therefore, it is unlikely thatdeprotonation and reprotonation in the epimerasereaction occur without the proton exchanging withbulk water. The NMR data strongly support the hypo-thesis ... if the reaction occurs in D2O [16]. Another possibility is that deprotonation and reprotonation occur without the proton exchanging with bulk water. However, de-protonation and reprotonation ... 100% proton occupancy at the 2¢ position,strongly suggesting that there is no deprotonation andreprotonation at C2¢ and the epimerase reaction fol-lows the aldol chemistry. Is the DHNA-catalyzed...

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