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Tài liệu Báo cáo khoa học: Characterization of chitinase-like proteins (Cg-Clp1 and Cg-Clp2) involved in immune defence of the mollusc Crassostrea gigas docx

Tài liệu Báo cáo khoa học: A novel c-N-methylaminobutyrate demethylating oxidase involved in catabolism of the tobacco alkaloid nicotine by Arthrobacter nicotinovorans pAO1 ppt

Tài liệu Báo cáo khoa học: A novel c-N-methylaminobutyrate demethylating oxidase involved in catabolism of the tobacco alkaloid nicotine by Arthrobacter nicotinovorans pAO1 ppt

... C-terminal domain of MABO to other proteins of the sarcosine dehydrogen ase and oxidase familymay indicate that this is the site of attachment of tetra-hydrofolate to the enzyme. c-Aminobutyrate ... generated during the catabolism of nicotine. The identification of this enzyme reveals, for the first time, the metabolic fate of the pyrrolidine ring of nicotine during the pAO1-dependent nicotine catabolism ... nicotine by A. nicotinovorans is linkedto the presence of the megaplasmid, pAO1 [20]. The recentelucidation of the DNA sequence of pAO1 revealed the modular organization of the enzyme genes involved...
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Tài liệu Báo cáo khoa học: Characterization of 1H NMR detectable mobile lipids in cells from human adenocarcinomas doc

Tài liệu Báo cáo khoa học: Characterization of 1H NMR detectable mobile lipids in cells from human adenocarcinomas doc

... spec-tra of cells has been studied subsequent to the firstobservations being made in cancer cells, lymphocytes and developing cells. On the other hand, a correlation of the intensity of these signals ... m2 and m3obtainedfrom fitting with Eqn (4) ML data, as in Fig. 5, for irradiated (I) and non-irradiated (C)HeLa and MCF-7 cells. The minimum values(tm) of the parabolic fitting curve and the corresponding ... PC and increased GPC, indicating an imbal-ance between PL synthesis and degradation and, con-sequently, an increase of TG. Other studies suggestthat the simultaneous accumulation of ML and...
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Tài liệu Báo cáo khoa học: Characterization of chitinase-like proteins (Cg-Clp1 and Cg-Clp2) involved in immune defence of the mollusc Crassostrea gigas docx

Tài liệu Báo cáo khoa học: Characterization of chitinase-like proteins (Cg-Clp1 and Cg-Clp2) involved in immune defence of the mollusc Crassostrea gigas docx

... Characterization of chitinase-like proteins (Cg-Clp1 and Cg-Clp2) involved in immune defence of the mollusc Crassostrea gigas Fabien Badariotti, Christophe Lelong, Marie-Pierre Dubos and ... the oyster Crassostrea gigas [5]. Interestingly, thisprotein, named C. gigas chitinase-like protein 1 (Cg-Clp1) was found to be involved in the control of growth and remodelling processes in ... of the soleGlyco_18 domain, the C-terminal tail of C. gigas CLPsmay not noticeably contribute to the structure and the function of these proteins. Interestingly, Cg-Clp1 and Cg-Clp2 C-terminal...
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Tài liệu Báo cáo khoa học: Characterization of human deoxyribonuclease I gene (DNASE1) promoters reveals the utilization of two transcription-starting exons and the involvement of Sp1 in its transcriptional regulation ppt

Tài liệu Báo cáo khoa học: Characterization of human deoxyribonuclease I gene (DNASE1) promoters reveals the utilization of two transcription-starting exons and the involvement of Sp1 in its transcriptional regulation ppt

... of alternative promoters in DNASE1 results in no generation of protein isoforms. The 5¢-UTR of eukaryotic mRNA in uences the initi-ation step of protein synthesis and thereby in partdetermines ... indicate intron 6 of 75 bp in fragmentsB and C, 3¢ portion of 35 bp in intron 5 in fragments C and D, and 3¢ portion of 25 bp in intron 6 in fragments F and I. The number at the right of each RT-PCR ... transcriptionfactor and related proteins, as shown in Fig. 1B. Toevaluate whether the Sp1-binding site in the DNASE1distal promoter is crucial for expression, a mutatedbinding site was introduced into the...
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Tài liệu Báo cáo khoa học: Characterization of the interaction between the plasma membrane H+-ATPase of Arabidopsis thaliana and a novel interactor (PPI1) doc

Tài liệu Báo cáo khoa học: Characterization of the interaction between the plasma membrane H+-ATPase of Arabidopsis thaliana and a novel interactor (PPI1) doc

... interacts with the first 88 aminoacids of PPI1 [22], indicating that the PM H+-ATPasebinding site of PPI1 is localized therein. Indeed, fusion proteins containing the first 88 amino acids of ... blue-lightactivation of PM H+-ATPase in guard cells of broadbeans involves protein kinase-mediated phosphoryla-tion of Ser and Thr residues in the C-terminus of the pump and 14-3-3 binding [17]. Much ... suggested by the parallel increase of the number of FC-bindingsites [18–20]. As to auxin, a soluble auxin receptor hasbeen reported to bind and activate the PM H+-ATPase,but the site of binding has...
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Tài liệu Báo cáo khoa học: Characterization of a recombinantly expressed proteinase K-like enzyme from a psychrotrophic Serratia sp. ppt

Tài liệu Báo cáo khoa học: Characterization of a recombinantly expressed proteinase K-like enzyme from a psychrotrophic Serratia sp. ppt

... (H) and Ser (S) are bolded; N-terminalresidues of the catalytic domain are under-lined. The preregion is indicated in red, the pro-region in black, catalytic domain in blue and the C-terminal ... suggest that the bands at 56 kDa and  45 kDa refer to a peptidase formincluding two C-terminal domains and one C-terminaldomain, respectively. The protein band at  34 kDarefers to the ‘mature’ ... One or more of the disulfide bondsis shown to be essential for maintaining the activeconformation of VPRK, since cleavage of the disul-fides lead to inactivation of the enzyme [25]. The first two...
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Tài liệu Báo cáo khóa học: Characterization of the products of the genes SNO1 and SNZ1 involved in pyridoxine synthesis in Saccharomyces cerevisiae pptx

Tài liệu Báo cáo khóa học: Characterization of the products of the genes SNO1 and SNZ1 involved in pyridoxine synthesis in Saccharomyces cerevisiae pptx

... Characterization of the products of the genesSNO1 and SNZ1 involved in pyridoxine synthesis in Saccharomyces cerevisiaeYi-Xin Dong, Shinji Sueda, Jun-Ichi Nikawa and Hiroki KondoDepartment ... the skeleton of pyridoxine in yeast[9,10]. Herein, we report that Sno1p and Snz1p serve as aglutaminase to supply ammonia for the ring nitrogen of pyridoxine in yeast. Based on these and other ... of the genes. In SNO1, the 199th G from the 5¢ terminus of the open readingframe was deleted to result in a frame-shift and appearance of stop codons in the downstream. As a result, a protein...
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Tài liệu Báo cáo khoa học: Characterization of promoter 3 of the human thromboxane A2 receptor gene A functional AP-1 and octamer motif are required for basal promoter activity docx

Tài liệu Báo cáo khoa học: Characterization of promoter 3 of the human thromboxane A2 receptor gene A functional AP-1 and octamer motif are required for basal promoter activity docx

... Transcriptional activation of the human hemato-poietic prostaglandin D synthase gene in megakaryo-blastic cells. Roles of the oct-1 element in the 5¢-flanking region and the AP-2 element in the untranslated ... FEBSExamination of the coordinate regulation of pro-moter 3 basal activity by the AP-1 and Oct-1/2transcription factorsTo determine the combined contribution of the AP-1 and Oct-1 ⁄ 2 cis-acting ... critical role in the assembly of the preinitiation complex withinTATA-less promoters [47]. The AP-1 complex is com-prised of a group of proteins encoded by the jun(c-Jun, JunB, JunD) and fos (c-Fos,...
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Tài liệu Báo cáo khoa học: Characterization of electrogenic bromosulfophthalein transport in carnation petal microsomes and its inhibition by antibodies against bilitranslocase docx

Tài liệu Báo cáo khoa học: Characterization of electrogenic bromosulfophthalein transport in carnation petal microsomes and its inhibition by antibodies against bilitranslocase docx

... cyanidin;100 nm bilirubin and 100 nm biliverdin. Under the condi-tions of the assay, bilirubin is freely soluble in the buffer[58]. The presence of these inhibitors in the assay mediummay interfere ... Number of [A] tested; k2⁄ k1, the value of the intercept in the Scrutton and Utter plot, where k2 and k1are the rate constants of the inhibition of either the bilitranslocase-ligand complex ... described in detail in both the experimental procedures(Ki of cyanidin, bilirubin, biliverdin, section B) and in Table 2 (Kd of the complexes of bilitranslocase with bilirubin, nicotinic acid and...
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Tài liệu Báo cáo khoa học: Characterization and mode of action of an exopolygalacturonase from the hyperthermophilic bacterium Thermotoga maritima doc

Tài liệu Báo cáo khoa học: Characterization and mode of action of an exopolygalacturonase from the hyperthermophilic bacterium Thermotoga maritima doc

... to be involved in substrate binding at subsite )2inE. caro-tovora EPG2, namely Arg152 binding the carboxygroup and Lys229 interacting with 2-OH, are also con-served in PelB and A. tubingensis ... that the intrinsic rate of hydrolysis (kint) in the productivecomplex is independent of the length of the substrate,Km and Vmaxwere used to calculate the subsite affinit-ies (see equations in ... fewinserted b-strands (1a in Fig. 2), and one large insert of 15 amino acids is present before the first b-sheet of coil 3, which is on the edge of the pronounced sub-strate-binding cleft in EPG2 (Fig....
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