Tài liệu Báo cáo khoa học: Caveolin-1 inﬂuences P2X7 receptor expression and localization in mouse lung alveolar epithelial cells docx

... of Caveolin-1 and P2X7distribution in control and in Caveolin-1 shRNA-transfected E10 cells. Note the loss of colocalization of Caveolin-1 and P2X7 in Cav-1 shRNA treated cells. Caveolin-1 and ... independentchannels (Fig. 5). Caveolin-1 and P2X7 receptor areendogenously expressed in E10 cells, and their localiza-tion in subconﬂuent cells was determined. Figure 5Ashows a Caveolin-1 staining pattern ... was also found in other pulmonary cells. shRNA-mediated downregulation and altered localization of Caveolin-1 in E10 cells To reduce the expression of Caveolin-1 protein in E10 cells, we designed...

Tài liệu Báo cáo khoa học: Functional hierarchy of plasminogen kringles 1 and 4 in ﬁbrinolysis and plasmin-induced cell detachment and apoptosis docx

... in plasmin formation, to a decrease in plasmin bindingor to a combination of the two. We demonstrate thatboth mAb 34D3 and mAb A10.2 inhibit plasminbinding to cells (Fig. 5A) and fibrin (Fig. 5B) in ... function. Blocking the kringle 1 lysine-binding site with monoclonal antibody 34D3 fully abolished binding and activation of Glu-plasminogen and prevented both fibrinolysis and plasmin-induced cell ... devel-opment and the absorbance at 405 nm was measured in amultiwell plate reader.To determine the effect of 34D3 and A10.2 mAbs onplasmin(ogen) binding to fibrin and CHO-K1 cells, fibrinplates and cells...

Tài liệu Báo cáo khoa học: Amprenavir complexes with HIV-1 protease and its drug-resistant mutants altering hydrophobic clusters docx

... Dashedlines indicate van der Waals contacts with interatomic distances in A˚. Dotted lines indicate C-HÆÆÆO interactions. Black lines indicate interac-tions in SQV complexes. Red lines indicate interactions ... hydrophobic interactions in proteins [26]. Removing a methyl group in the proteinhydrophobic core affects protein folding and decreasesthe protein stability in mutant proteins [27]. In PR,two ... Exploring the changes in PR due tobinding of two different inhibitors will give insight intothe mechanisms of resistance and help in the design ofnew inhibitors.ResultsAPV inhibition of PR and...

Tài liệu Báo cáo khoa học: A strategy for discovery of cancer glyco-biomarkers in serum using newly developed technologies for glycoproteomics ppt

... stromal cells are difﬁ-cult to distinguish from those involved in wound heal-ing and in ammation. In association with cancergrowth, the stromal cells grow and expand to releasemany glycoproteins ... have included a full list of N-glycopro-teins from C. elegans and a partial list from mouse livercontaining the protein (gene) ID, protein name, glycosy-lated sites, and kinds of lectins used ... candidates, i.e. core proteins that carry anepitope bound by a speciﬁc lectin (stage III). Theabundance of each glycoprotein in serum was esti-mated by IGOT using Ricinus communis agglutinin...

Tài liệu Báo cáo khoa học: Synchronization of Ca2+ oscillations: involvement of ATP release in astrocytes pdf

... 2009)doi:10.1111/j.1742-4658.2009.07438.xGlial cells, especially astrocytes, are not merely supportive cells, but areimportant partners to neighboring cells, including neurons, vascular cells, and other glial cells. Although glial cells ... fromastrocytesThe P2 receptors responsible for increases in [Ca2+]i in astrocytes are well characterized, and G-protein-cou-pled P2Y1 and P2Y2 receptors have been shown toplay central roles in the event ... astrocyticCa2+transients are accentuated in pathological con-ditions, suggesting the involvement of astrocytic Ca2+transients in brain disorders, including epileptic sei-zures. Interestingly, astrocytes also...

Tài liệu Báo cáo khoa học: The central role of CDE/CHR promoter elements in the regulation of cell cycle-dependent gene transcription pdf

... theMuvB proteins Lin-37, Lin-52, Lin-54 and chromatin-associated Lin-9 and Lin-53 ⁄ RBBP4 ⁄ RbAp48.Furthermore, A-Myb and B-Myb were present in immunoprecipitations for Lin-9, Lin-37 and Lin-54[90,92]. ... such as cyclinA and cyclin B1, in dividing cells [92]. Furthermore,depletion of Lin-9 and B-Myb in p53) ⁄ )MEFs causedlowered expression of cyclin A, cyclin B and Cdc2 [94]. In similar experiments, ... ﬁrst observed in the Cdc25C pro-moter by in vivo footprinting as being protected in G0 cells. The Cdc25C gene is not expressed in resting cells or in cells in G1phase. Only in G2phase can...

Tài liệu Báo cáo khoa học: Accessibility changes within diphtheria toxin T domain when in the functional molten globule state, as determined using hydrogen⁄deuterium exchange measurements pdf

... chaincorresponding to the catalytic domain (C domain); and the B chain corresponding to the translocationdomain (T domain) and the receptor- binding domain.The C and T domains remain covalently linked ... Following binding to its cell-surface receptor, diphtheria toxin is internalized through theclathrin-coated pathway. The acidic pH in the endo-some triggers a conformational change, leading toinsertion ... pro-tein in the N- and MG states, the regions of moderate and high accessibility, and regions involved in the olig-omerization of both states of the T domain in solution.ResultsMonitoring H⁄D-exchange...

Tài liệu Báo cáo khoa học: Exposure of IgG to an acidic environment results in molecular modiﬁcations and in enhanced protective activity in sepsis doc

... lgÆmL)1(lines 4–6). (C) Increased binding of pH 4 buffer-exposed IVIg to recombinant human IFN-c. (D) Increased binding of low-pH buffer-exposed IVIg to human factor H. In both panels, bindingof ... antigen-bindingspeciﬁcities (Fig. 1B).Enhanced binding of low-pH buffer-exposed IVIgto recombinant human interferon-c (IFN-c) and other self-antigensInteractions of IVIg with cytokines and other ... interaction in the presence of increasing concentrations of NaCl. In both experiments, binding intensities are represented in RU, and each data point shows the mean value ± standard deviation of quadruplicate...

Tài liệu Báo cáo khoa học: Disulﬁde bridge regulates ligand-binding site selectivity in liver bile acid-binding proteins ppt

... changes in ligand-binding stoichiometry and affinities. The removal of adisulfide bond in rat lipocalin-type prostaglandin Dsynthase slightly increased the binding affinity for bio-logical ligands, ... uponligand binding. Indeed, high T1⁄ T2ratios wereobserved for Tyr9 and Gln11 (strand A), Arg32 (helixII), Val90, Lys92, and Glu94 (strand G), Phe96 and Ser97 (strand H), Phe113 (strand I), and ... leadto distinct binding and functional properties [22,23]. In line with this, we have shown here that the presence ofa disulfide bridge, while maintaining the same bindingstoichiometry, induces...

Tài liệu Báo cáo khoa học: Properties of ecdysteroid receptors from diverse insect species in a heterologous cell culture system – a basis for screening novel insecticidal candidates docx

... fordeveloping a screening system to identify and charac-terize candidate insecticidal compounds showing bothinductive and potentiative activity.ResultsThe DNA-binding domains (DBDs) of Leptinotarsa and ... linker region and ligand-binding domain (LBD) of ecdysone receptors.Fig. S2. Amino acid alignment of linker region and ligand-binding domain (LBD) of ultraspiracle ⁄ RXRreceptors.This supplementary ... Howell L, Hannan GN & Hill RJ (2007)Ligand binding by recombinant domains from insectecdysone receptors. Insect Biochem Mol Biol 37, 611–626.12 Minakuchi C, Ogura T, Miyagawa H & Nakagawa...

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