Tài liệu Báo cáo khoa học: The stereochemistry of benzo[a]pyrene-2¢-deoxyguanosine adducts affects DNA methylation by SssI and HhaI DNA methyltransferases pptx

... with the levels of DNA methylation, and thus the effect of the abso-lute conﬁgurations and conformations of the adducts on DNA methylation was not evaluated. Morerecently, the effect of stereochemically ... (2007) 2121–2134 ª 2007 The Authors Journal compilation ª 2007 FEBS The stereochemistry of benzo[a]pyrene-2¢-deoxyguanosine adducts affects DNA methylation by SssI and HhaI DNA methyltransferases Oksana ... duplexalone and in complexes with MTases, the ﬂuorescenceintensities at 384 nm were calculated.Determination of the amounts of the active form of M .SssI and M .HhaI The amount of the active form of M.SssI...

Tài liệu Báo cáo khoa học: The occurrence of hemocyanin in Hexapoda docx

... mediated by the tracheal system, a network of tubules that open to the atmosphere on the cuticle and radiate to all parts of the body. O2is delivered through trachea and trache-oles in the gaseous ... indication of the presence of hemocyanin(data not shown). The notion of the absence of hemo-cyanins from Holometabola is corroborated by the factthat no hemocyanin sequences could be identiﬁedin the ... possible ‘intermediate’. The evolution of hemocyanin subunits follows the widely accepted phylogeny of the Hexapoda and provides strong evidencefor the monophyly of the Polyneoptera (Plecoptera,...

Tài liệu Báo cáo khoa học: The effect of small molecules in modulating the chaperone activity of aB-crystallin against ordered and disordered protein aggregation pdf

... (seebelow). The effect of the additives on aggregation of the target protein (in the absence and presence of aB-crystallin)was assessed at the end of each assay by calculating the percentage ... aB-crystallin (i.e. the effect of the additive on the aggregation of the target protein is inde-pendent and different from that of the chaperone). The standard errors associated with this theoretical ... calculated from the standard errorsassociated with the means of the measured values (i.e. by calculating the square root of the sum of the associatedindividual errors squared). The maximum percentage...

Tài liệu Báo cáo khoa học: The sequentiallity of nucleosomes in the 30 nm chromatin ﬁbre pptx

... times of digestion, morethan 50% of the sample was converted into the frac-tion S. Breaking the disulfate bond in the middle of the crosslinker produced only mononucleosomes and the band S. DNA ... rest, even by the zero-length crosslinker, perhaps via linker histones H1 and H5. Indeed, there is evidence that the tails of thesehistones follow the path of the linker DNA [5]. Theyare crosslinked ... they consider the topological constraintsimposed by the length of the linker DNA on the tilts of the nucleosomes, as discussed elsewhere [24].Dorigo et al. [14] studied the sequentiallity of the...

Tài liệu Báo cáo khoa học: The localization of FGFR3 mutations causing thanatophoric dysplasia type I differentially affects phosphorylation, processing and ubiquitylation of the receptor pptx

... prevented by blocking transport from the ER to the Golgi. BFA had no effect on the relativelack of the 130 kDa isoform of the K650M mutant.Endo H digestion of the immunoprecipitated wild-type and ... and located in different domains of the recep-tor (Fig. S1) was created by site-directed mutagenesis of the full-length human FGFR3 cDNA and subclon-ing into the pcDNA3.1 vector. Based on the ... role for components of the ER–Golgi vesicle transport. Through the use of mark-ers for the ER (PDI) and the Golgi apparatus(GM130, p230), the phosphorylated isoforms of the K650M mutant were...

Tài liệu Báo cáo khoa học: The role of electrostatic interactions in the antitumor activity of dimeric RNases docx

... electrostatic ﬁeld of differentFig. 5. Plot of the EIE values as function of rotation (r) and inclination (i) angles of RNases. The hatched axis at the bottom and right of the plots show schemes of some ... DMPG membranes, as evidenced by a decrease of the amplitude of their thermotropictransition. Only RNase A and RNase AA show noeffects on the thermotropic behavior of the testedmembranes. Surprisingly, ... regions,respectively, of the two A and B monomers of the dimeric RNase. The black circle between the monomers indicates the mass center of the dimer.E. Notomista et al. Electrostatic interactions and antitumor...

Tài liệu Báo cáo khoa học: The role of antioxidants in the cytotoxicity of chemotherapeutic drugs pptx

... from the deleterious effects of DNA injury a net-work of genome care-taking mechanisms has evolved of which aset of DNA repair systems constitute a key component. The clinical impact of the DNA ... undergoes fusion with the membrane of intra-endosomal vesicles contained within MVBs,thereby releasing the nucleocapsid into the lumen of these vesi-cles. Then, the capsid uses these intra-endosomal ... metabolic syndrome and type 2 diabetes. Thispresentation will highlight the molecular mechanisms of FXR and PPARalpha action in the prevention and treatment of the metabolic syndrome and atherosclerosis.3.5...

Tài liệu Báo cáo khóa học: The effect of mutations surrounding and within the active site on the catalytic activity of ricin A chain pptx

... Electron density of RTA R213D in the vicinity of residue 213. The backbone and sidechains of the R213D substitution are shown as stereoimages in thick ball and stick and the position of the Arg213 ... by the addition of 100 lLof2· Kirby buffer [25] and 80 lLofH2O, and rRNA was obtained by precipitation after two phenol–chloroform extractions. Four micrograms of rRNA weretreatedwith20lL of ... levels and, as such, these mutantscould not be puriﬁed. To assess the effect of substitutionsmade at each of the arginyl residues on catalytic activity of RTA, the ability of each of the puriﬁed...

Tài liệu Báo cáo khóa học: The lysozyme of the starfishAsterias rubens A paradigmatic typei lysozyme docx

... the lysozyme of the bivalve Tapes japonica [3], with the so-called chlamysin of Chlamys islandica [4,5], and also with a hypotheticalsecreted protein of the nematode Caenorhabditis elegans and with ... between this plot and the corresponding part of the HCA plot of the Asteriaslysozyme. The same overlap is obtained when comparing the human lysozyme and/ or the chicken lysozyme with the protostome ... Putative three-dimensional model of the Asterias lysozyme i. These ﬁgures were generated with the help of the SWISS-PDBVIEWERsoftware. (A)ApartoftheputativestructureoftheAsterias lysozyme, from...

Tài liệu Báo cáo khoa học: The role of the ESSS protein in the assembly of a functional and stable mammalian mitochondrial complex I (NADH-ubiquinone oxidoreductase) pptx

... revealed the existence of two additionalsubunits (bringing the total to 46), and one of these, the ESSS protein, is also encoded by an X-linked g ene inhumans and mouse [36]. Most of these subunits ... the level of a complex of 46 subunits isdetermined. The activities of complex II and the downstream complexIII of the electron transport chain were measured and foundto be near normal in the ... culateabout the formation of supercomplexes and the effect of the absence of intact complex I, but the results are toopreliminary in this regard.Heterologous expression of the ESSS-HA subunit The localization...

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