Tài liệu Báo cáo khoa học: Implication of the glutamine synthetase ⁄glutamate synthase pathway in conditioning the amino acid metabolism in bundle sheath and mesophyll cells of maize leaves doc

... 2008 The Authors Journal compilation ª 2008 FEBS Implication of the glutamine synthetase ⁄glutamate synthase pathway in conditioning the amino acid metabolism in bundle sheath and mesophyll cells ... 33% in the light to 25% in the dark (Fig. 7A). Glutamine and asparagine were the major amino acids in the xylem sap in both the light(63% of the total amino acids) and dark (60%)(Fig. 7B). The ... and reassimilation into amino acids between bundle sheath cells and mesophyll cells for the remo-bilization of amino acids during the early phase of grain ﬁlling in Zea maysL. The plants responded...

Tài liệu Báo cáo khoa học: Motion of the Ca2+ -pump captured ppt

... (long-distance move-ment of the N-domain and rotational motion of the A-domain) of SERCA during the ATP-mediated iontransport reaction [14,16–19]. The single line scanning method [8,15], in which anAFM ... accu-mulates Ca2+inside the SR against its concentrationgradient. The importance of the SR Ca2+-pump wasrealized in the early 1960s by Ebashi and Lipmann[5,6] and, since then, most of the molecular ... to the heightdifference between the E1Ca2+form [14], in which the N-domain is widely separated from the A-domain and P-domain, and the other compacted forms of SERCA (E1ATP, E1P, E2P, and...

Tài liệu Báo cáo khoa học: Role of Kupffer cells in pathogenesis of sepsis-induced drug metabolizing dysfunction pptx

... mediator of lethal sepsis, triggering the production of proinﬂammatory cytokines and liverinjury [13].Therefore, the present study aimed to elucidate the role of KCs in the regulation of CYP isoform ... Binding of all of the antibodies was detected using anECL detection system (iNtRON Biotechnology) in accor-dance with the manufacturer’s instructions. The intensity of the immunoreactive bands ... constitutes one of the major aspects of hepatocyte function and contributes to the metabolism and elimination of exogenous and endogenous sub-stances [4]. In various models and in clinical reports,KeywordsCYP450;...

Tài liệu Báo cáo khoa học: Mechanisms of amyloid ﬁbril formation – focus on domain-swapping doc

... ﬁbril building blocks only after partial unfold-ing of the a-helix from the body of the b-sheet(Fig. 2C).Possible role of domain-swapping in the ‘off -pathway folding’ (OFF) model The OFF model ... asparagine-rich regions are commonlyfound in the N-termini of both mammalian and yeastprion proteins [96] and several other proteins withpolyglutamine expansions such as huntingtin and ataxin-3. The ... and Murphy [167]. The generalmodel is described in more detail in Fig. 1F and isincluded in Table 1. The off -pathway oligomers are the dead end of an alternative folding pathway, which isincapable...

Tài liệu Báo cáo khoa học: Modulation of a-synuclein aggregation by dopamine in the presence of MPTP and its metabolite pptx

... 1B shows the patternseen after the development of the blot. With increase in time of incubation, the intensity of the band for the monomeric protein decreased, whereas the bands for the higher ... line), in the presence of 100 lM neurotoxin (•, dotted line), in the presence of 200 lM neurotoxin ( , dottedline) and in the presence of neurotoxin and 50 lM dopamine (h, dashed line).Modulation ... solu-tion of ThT and the ﬂuorescence intensity of the solu-tion was measured at 482 nm. Figure 4A shows the kinetics of aggregation of a-synuclein in the presence of 100 lm MPTP and the effect of 50...

Tài liệu Báo cáo khoa học: Modulation of F0F1-ATP synthase activity by cyclophilin D regulates matrix adenine nucleotide levels pptx

... CYPD binding to the innermembrane by means of the decreasing matrix Picon-centration, which in turn could affect CYPD bindingto F0F1-ATP synthase, and decreased binding of the inhibitory ... size (cut-off of $ 1.5 kDa) to allow the pas-sage of solutes and water, which may also result in rupture of the outer membrane. The identity of the proteins comprising the PTP is debated; the ubiqui-tous ... signiﬁcant production of Pi in the vicinity of the ATPase within the matrix.CYPD ablation or its inhibition by cyclosporin Aincreases the rate of respiration stimulated byarsenate in intact mitochondriaRegarding...

Tài liệu Báo cáo khoa học: Role of the cag-pathogenicity island encoded type IV secretion system in Helicobacter pylori pathogenesis pptx

... distribution,making them unsuitable for the investigation of b-cate-nin signalling [29]. In support of this aspect, usingMadin–Darby canine kidney cells that expressE-cadherin and b-catenin without ... control the amount of intracellu-lar CagAPY. Cortactin, ezrin and vinculin are all Srcsubstrates, and Src inactivation causes dephosphoryla-tion of these proteins and is crucial for triggering ... hallmark of the ulceration process, HtrA cleaves the junctional protein E-cadherin [8]. Another intriguingpossibility of junction disruption could be the transcytosis of basal integrins to the apical...

Tài liệu Báo cáo khoa học: Interruption of triacylglycerol synthesis in the endoplasmic reticulum is the initiating event for saturated fatty acid-induced lipotoxicity in liver cells pdf

... (Fig. 5B). The ER is the site of synthesis of all secretory pro-teins and resident proteins of the membrane system, and any perturbation that compromises the protein-folding capacity of the organelle ... synthesiswas initially hindered following stearate administration and was completely interrupted at longer incubationperiods. Interestingly, the interruption of TAG synthe-sis preceded the ... in metabolism of FFAs aftertheir uptake into liver cells is the long-chain acyl-CoA synthetase (ACS), which activates fatty acids by link-ing them to coenzyme A. As shown in Fig. 6, triac-sin...

Tài liệu Báo cáo khoa học: Kinetics of dextran-independent a-(1 ﬁ 3)-glucan synthesis by Streptococcus sobrinus glucosyltransferase I pdf

... in the intermediate oligosaccharides during the glucan syn-thesis can be interpreted with respect to the kineticproperties of enzymes. In other words, the relationshipbetween the enzyme kinetic ... properties and the process of insoluble glucan synthesis remains unknown.To address these issues, we analyzed the process of dextran-independent insoluble glucan formation and examined the enzyme kinetic ... respectively. They share highlyhomologous amino acid sequences and comprise the N-terminal glucansucrase domain (GSd) and the C-terminal glucan-binding domain (GBd) [3,4]. The GSd catalyzes glucosyl transfer...

Tài liệu Báo cáo khoa học: Regulation of DNA fragmentation: the role of caspases and phosphorylation doc

... protein, the degree of activation and the in uence of other proteins. Phosphorylation signaling pathways are intri-cately interrelated; it was previously shown that either induction or inhibi-tion ... Pro-caspases exist in living cells and casp-ases are indispensable for the proliferation of some celllines. When deciding between maintaining cells alive orinducing apoptosis, caspase function and activationare ... is under the direct control of kinases and phosphatases, and the indirect control of phosphorylation through the regula-tion of other apoptotic proteins. Furthermore, manykinases and phosphatases...

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