Tài liệu Báo cáo khoa học: Identiﬁcation of differentially expressed genes of the Paciﬁc oyster Crassostrea gigas exposed to prolonged thermal stress docx

... claim to original French government works Identification of differentially expressed genes of the Pacific oyster Crassostrea gigas exposed to prolonged thermal stress Anne-Leila Meistertzheim1, Arnaud ... constitutive isoforms of HSP70 aresynthesized in the gills and mantle of C. gigas [26]. The expression level of the constitutive forms increasesafter thermal stress, whereas the inducible one is expressed ... the area. Our study is the first to applyan overall genomic approach to the study of the response of C. gigas to prolonged heat stress. We usedanimals outside the season of reproductive develop-ment...

Báo cáo khoa học: Molecular identiﬁcation and expression study of differentially regulated genes in the Paciﬁc oyster Crassostrea gigas in response to pesticide exposure doc

... less than the concentrations introduced in the tanks, probably due to adsorption of atrazine to the surfaces of the tanksand possibly due to removal by the fish themselves[46]. Moreover, the authors ... contaminants.Most of the genes we identified function in the res-piratory chain, cell communication, the immune sys-tem, or the regulation of protein or the cytoskeleton.Only a few are specific to xenobiotic ... mgÆL)1. The supposedlow level of toxicity of glyphosate to oysters, and the weak concentration used in our experiment couldpartly explain the difference observed in the number of genes identified...

Tài liệu Báo cáo khoa học: Identiﬁcation of Ewing’s sarcoma protein as a G-quadruplex DNA- and RNA-binding protein ppt

... aimed to determinewhether RGG3 of EWS modulates the formation orunwinding of Htelo G-quadruplex DNA. To determinewhether the RGG3 binding affected the stability of the G-quadruplex structure of ... synthetic oligonucleotides andthus appears to recognize the G-quadruplex DNAstructure independently of the sequence context.We further investigated the region of EWS that con-tributes to the ... RNA in vitro [8]. On the other hand, Hume et al. [15] suggested that EWSbinds to the proximal-element DNA of the macro-phage-speciﬁc promoter of the CSF-1 receptor gene. The RGG domain, initially...

Tài liệu Báo cáo khoa học: Identiﬁcation and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebraﬁsh, Danio rerio docx

... relationshipbetween the expression of these transcription factorsand the cytokine genes known to be produced by dif-ferent T-cell subtypes in mammals. These transcriptionfactors, together with many of the ... whether these cytokines areproduced by CD4+CD25+Foxp3+Tregor whetherthey induce the production of these cytokines fromanother population of cells [20]. To date, our knowledge about the ... regula-tor of Th1 differentiation [11]. T-bet potentiates the expression of IFN-c, which, in turn, upregulates the inducible chain of the IL-12 receptor (IL-12Rb2).Binding of IL-12 to its receptor...

Tài liệu Báo cáo khoa học: Identiﬁcation of two late acyltransferase genes responsible for lipid A biosynthesis in Moraxella catarrhalis doc

... found the lgt5gene encoding an a-galactosyltransferase for addition of the terminal galactose of the LOS [25], and Schwin-gel et al. found the lgt6 gene involved in the initialassembly of the ... constructed the corresponding isogenic mutants. Analysis of physio-chemical and biological features of both mutants wasperformed to study the functions of these genes and the structures of their ... hydrolysis of bacterial endotoxins and determina-tion of the anomeric conﬁguration of the glycosylphosphate present in the ‘isolated lipid A’ fragment of the Bordetella pertussis endotoxin. Carbohydr...

Tài liệu Báo cáo khoa học: Identiﬁcation and characterization of an R-Smad ortholog (SmSmad1B) from Schistosoma mansoni pdf

... domain,causing the R-Smad to dissociate from the receptor. The phosphorylation ⁄ activation of the R-Smad allowsit to interact with another component of the Smadfamily, called a common Smad (Co-Smad). The ... FEBS The protein localization of SmSmad1B in the sub-tegument of adult schistosomes is a common expres-sion pattern among the schistosome Smads. The presence of SmSmad1B in the subtegument furthersupports ... per reaction), and, in the case of SmSmad2, the act-ive mutant construct of type I receptor, SmTbRI-QD (10 lL). The top arrow points to SmSmad4, and the bottom arrow points to SmSmad2in vitro...

Tài liệu Báo cáo khoa học: Identiﬁcation, sequencing, and localization of a new carbonic anhydrase transcript from the hydrothermal vent tubeworm Riftia pachyptila docx

... wasobserved in the cytoplasm of the cells. The stainingappears to be speciﬁc of the probe sequence because the staining is clear with the complementary sequence to RpCAbr, but not with the sense ... detection of RpCAtr mRNA in both the epidermal cytoplasm of the branchial ﬁlaments andin the cytoplasm of the trophosomal bacteriocytes. Wecould only detect RpCAbr mRNA in the epidermalcytoplasm of ... of the CA D. melanogastersequences and, on the other hand, the otherD. melanogaster sequences more closely related to the CAVI vertebrate isoforms (CAa, CAb and D. melano-gaster-3) or to the...

Tài liệu Báo cáo khoa học: Identiﬁcation of a novel matrix protein contained in a protein aggregate associated with collagen in ﬁsh otoliths pdf

... the potential function of OMM-64. In the inner ear sacculus, the otolith is close to the proximalside of the sacculus, and calcification of the otolithoccurs mainly at the proximal surface [19]. ... notbe confirmed to be otolin-1. Therefore, MALDI-TOF-TOF tandem mass spectrometry was performed to identify the proteins. The tryptic peptide mass finger-printing spectra of these proteins were ... matrix proteins, otolithmatrix protein-1 and otolin-1, are also localized inthese cells [11]. Similar to the other otolith proteins,OMM-64 may contribute to the heterogeneity of the endolymph...

Tài liệu Báo cáo khoa học: Identiﬁcation of b-amyrin and sophoradiol 24-hydroxylase by expressed sequence tag mining and functional expression assay docx

... biosynthesis of triterpenesaponins is required, including the enzymes catalyzing the sequence of reactions and the genes encoding theseenzymes. The biosynthesis of triterpene saponins involves the initial ... void vector). The MS fragmentation pattern wasalso identical to that of the authentic olean-3b,24-di-acetoxy-12-ene, except for the presence of several back-ground peaks (the amount of authentic ... contribute to the skeletal diversity of triterpenes. Subsequent regio- andstereospeciﬁc hydroxylations of the skeleton producefurther structural diversity. In contrast to the rapidprogress in the...

Tài liệu Báo cáo khoa học: Identiﬁcation of GAS-dependent interferon-sensitive target genes whose transcription is STAT2-dependent but ISGF3-independent doc

... out MatInspector analyses (seebelow) of the upstream promoter regions of IFN-regu-lated genes to determine the presence of ISRE, GASand additional regulatory sequences. Among the genes selected ... expression inresponse to IFN, conﬁrming the importance of STAT2function to ISG expression. Furthermore, several genes known to be important for mediating the biolo-gical effects of IFN, most notably ... JT, et al. (2000)Gene ontology: tool for uniﬁcation of biology. The Gene Ontology Consortium. Nat Genet 25 25–29], asshown on the legend. Most of the proteins fall into cel-lular fate and organization,...

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