Tài liệu Báo cáo khoa học: Metabolic gene switching in the murine female heart parallels enhanced mitochondrial respiratory function in response to oxidative stress pdf

... Metabolic gene switching in the murine female heart parallels enhanced mitochondrial respiratory function in response to oxidative stress M. Faadiel Essop1,2, W. Y. A. Chan2and Heinrich ... Discussion The main finding of this study is the identification of a metabolic gene switch from fatty acids to glucose in the murine female heart at baseline linked to enhanced mitochondrial respiratory ... utili-zation by the diabetic heart [9]. To functionally assess the significance of thesefindings, we evaluated mitochondrial respiratory function at baseline and in response to acute oxygenABCDEFFig....

Tài liệu Báo cáo khoa học: Globin gene family evolution and functional diversiﬁcation in annelids ppt

... marina Mb, (d) the gene structure of the new intra-cellular circulating globin from Al. pompejana, (e) the gene structure of intracellular circulating globin fromG. dibranchiata, and (f) the gene ... (proteinsinvolved in the structure of HBL-Hb) to interact and to bind to dimers or other aggregation states while conser-ving functionality. Indeed, linkers are thought to resultfrom duplication of globins ... comprises all intracellu-lar globins and the other all the extracellular globins. The intracellular cluster includes the nerve myoglobin(nMb) and all Mbs and intracellular Hbs. The extra-cellular...

Tài liệu Báo cáo khoa học: Metabolic control of mitochondrial properties by adenine nucleotide translocator determines palmitoyl-CoA effects Implications for a mechanism linking obesity and type 2 diabetes pdf

... 56% of the increase in the QH2⁄ Qratio, 70% of the increase in Dw, 72% of the increase in the ATP in ⁄ ADP in ratio, and 59% of the decrease in the ATPout⁄ ADPoutratio with succinate as ... Palmitoyl-CoAtended to increase the positive control of the ATP in ⁄ADP in ratio by ATP synthesis and the negative controlby the proton leak. The negative control of the ATP in ⁄ADP in ratio by the ANT increased ... succinate, control of the ﬂuxes shifts from the respiratory chain to ATP synthe-sis. Furthermore, the distribution of the control within the respiratory chain shifts from the part downstreamof...

Tài liệu Báo cáo khoa học: Effect of deletion of the DNase I hypersensitive sites on the transcription of chicken Ig-b gene and on the maintenance of active chromatin state in the Ig-b locus docx

... was introduced into DT40cells. Ig-b gene expression, acetylation of H3 and H4histones, and the remaining DHSs in the deleted cellswere examined to determine the roles of these DHSson the ... regions having transcriptionalenhancing activity were found in the intergenic regionbetween the Ig-b and GH genes. A member of the OCT family transcription factors appeared to beinvolved in the transactivation ... ﬁrst intron of the Ig-b gene. Furthermore, the acetylation status of H3 andH4 histones was examined [15]. In the present study,10 DT40-speciﬁc DHSs found in the Ig-b locus weredivided into four...

Tài liệu Báo cáo khoa học: Metabolic ﬂux proﬁling of Escherichia coli mutants in central carbon metabolism using GC-MS docx

... unex-pected ﬂux responses that provide new insights into the behavior of the metabolic network in its entirety. Mostprominently, the low in vivo activity of the Entner–Doudoroﬀ pathway in wild-type ... histidine, isoleucine, leucine, phenyl-alanine, proline, serine, threonine, tyrosine, and valine for[U-13C]glucose experiments and aspartate, isoleucine, leu-cine, phenylalanine, serine, ... substrates. To obtain the exclusive mass isotope distribution of the carbonskeleton, MDVawere corrected for the natural isotopeabundance of O, N, H, Si, S, and C atoms in the derivatizingagent...

Tài liệu Báo cáo khoa học: Regulation of DNA fragmentation: the role of caspases and phosphorylation doc

... FEBSenabling multiple mitochondrial proteins to be releasedinto the cytosol with cytotoxic activities [85]. The activated BH3-only protein Bad is also localizedmostly in the cytosol in normal ... site for the survival function of Bcl-2 [82]. The pro-apoptotic Bcl-2 proteins relocate to the sur-face of mitochondria during apoptosis. They induce the permeabilization of the mitochondrial ... membranewith the release of cytochrome c and the formation of the apoptosome [38]. The major pro-apoptotic proteinBax exists mainly in the cytosol or loosely attaches to mitochondria in an inactive...

Tài liệu Báo cáo khoa học: Basis of recognition between the NarJ chaperone and the N-terminus of the NarG subunit from Escherichia coli nitrate reductase pdf

... details about the interaction, ITC wasused to monitor the binding of the NarG peptides to NarJ. Surprisingly, the binding isotherm was biphasic,with the best ﬁt obtained with a two binding sitemodel, ... coupled to the absorption or release of protonsby the protein or the ligand. If this is the case, the bind-ing enthalpy is dependent on the ionization enthalpy of the buffer in which the reaction ... hydrogen bonds. Interestingly,this biphasic behaviour disappeared by increasing the pH, suggesting a protonation event. At pH 8, the binding isotherm generated a sigmoidal binding curvethat reached...

Tài liệu Báo cáo khoa học: An autoinhibitory effect of the homothorax domain of Meis2 ppt

... similar-ity) over the 132 amino acid domain C-terminal to the HD in Meis2d. We therefore suggest that the autoin-hibitory function of the Hth domain in Meis2d is likely to be a common feature ... proteins bind their cognate DNA-bind-ing site, they generally bind to other DNA-bindingcofactors [10–12]. Meis2 is a member of the TALEsuperfamily of HD proteins, which are characterizedby the ... negative. The Hth domain inhibits the activity of a linkedAD To further delineate the region required for the inhibi-tory effect of the Hth domain, we created a series ofGDB fusion proteins (Fig....

Tài liệu Báo cáo khoa học: Steﬁn A displaces the occluding loop of cathepsin B only by as much as required to bind to the active site cleft doc

... withchagasin and steﬁn A shows that the magnitude of the shift of the occlud-ing loop is related to the size of the binding region. It is smallest in the procathepsin structures and increases in the ... cleft(Fig. 2). These additional features hinder binding along the whole interdomain interface, although they bothare displaced upon binding of the ligand. The N-terminal trunk and the ﬁrst binding loopocclude ... competewith the occluding loop for binding to the active site, the farther away the occluding loop residues areshifted. As seen in the tetragonal form of the cathepsinB chagasin complex (3CBK), the...

Tài liệu Báo cáo khoa học: Functional characterization of an orphan cupin protein from Burkholderia xenovorans reveals a mononuclear nonheme Fe2+-dependent oxygenase that cleaves b-diketones ppt

... byxanthine oxidase was followed indirectly by measuring the increase in absorbance at 550 nm resulting from cyto-chrome c being reduced by superoxide in the course of the reaction. The principle ... ligands.Note that the coordinating histidines are contributedfrom b-strands of the central cupin barrel, suggesting arather rigid metal-binding site. Residues in the immedi-ate vicinity of the metal ... Germany). The pTYB1 vector harboring the target gene was transferred into the expression strainE. coli BL21(DE3). It encodes a chimeric form ofBxe_A2876 that has the IMPACT tag fused to the authenticC-terminal...

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