Tài liệu Báo cáo khoa học: Differential effects of Mxi1-SRa and Mxi1-SRb in Myc antagonism ppt

... predominanttranscript in the adult intestine and in the developingembryo, whereas Mxi1-SRb transcripts predominate in the adult liver and kidney [12]. In our initial description of Mxi1-SRa and ... arising in the REF assay. E1 is from a Myc + Ras +empty vector point, a1 and a2 are from Myc+ Ras+ Mxi1-SRa points, and b1 and b2 are from Myc+ Ras+ Mxi1-SRb points. TheSRa -myc and SRb -myc ... overexpressing Mxi1-SRa- myc and Mxi1-SRb- myc, respectively. The blot was probed with Myc tag antibody. The arrowindicates the Mxi1-SRa- myc protein observable in established celllines derived from Myc+ ...

Tài liệu Báo cáo khoa học: Molecular evolution of shark and other vertebrate DNases I pptx

... mice have an increased incidence of systemic lupus erythematosus (SLE), with classical ﬁndingsincluding the presence of autoreactive antibodies and glomerulonephritis occurring in a DNase I-level-dependentmanner; ... previously.Proteins were determined using a protein assay kit (Bio-Rad) with BSA as a standard. SDS/PAGE was performed in 12.5% (w/v) gels according to the method of Laemmli[24], and the proteins thus ... UÆmL)1penicillin, 50 lgÆmL)1streptomycin and 1 0% (v/v) fetalbovine serum (Invitrogen) at 37 °C under 5% (v/v) CO2 in air. The cells were transiently tran sfected using Lipofecta-minPlus reagent...

Tài liệu Báo cáo khoa học: Structural effects of a dimer interface mutation on catalytic activity of triosephosphate isomerase The role of conserved residues and complementary mutations pptx

... important in promoting subunit dissocia-tion [27] and also in maintaining the geometry of theactive site. The availability of crystal structures of TIMs from 21 sources and the large database of TIMsequences ... chain of the tworesidues brings the guanidinium plane and the aro-matic ring of F102 into close proximity, with analmost perfectly parallel arrangement of the interactinggroups (Fig. 9A). Interactions ... necessarily result in displacement of the F102 side chain, with consequent effects on interactions involving R98.Modeling studies indicated that insertion of a Trp res-idue at position 74 in the PfTIM...

Tài liệu Báo cáo khoa học: Antimicrobial effects of H4-(86–100), histogranin and related compounds – possible involvement of DNA gyrase ppt

... processed into buforin-I and fixed to the mucous surface of epithelial cells toprevent the entry of bacteria into the epithelial layer and their invasion into the lower parts of the gastro-intestinal ... with those of ciprofloxacin, LL-37 and amp-icillin (an antibiotic acting as an inhibitor of cell wallsynthesis) indicated that the inhibitory effect of thepotent quinolone ciprofloxacin was mimicked ... penetrating their membrane and binding to the DNA without inducing cell lysis. In the present study, HNr and H4-(86–100), like thequinolone antibiotic ciprofloxacin, displayed rapid(within 30 min;...

Tài liệu Báo cáo khoa học: Differential expression of duplicated LDH-A genes during temperature acclimation of weatherﬁsh Misgurnus fossilis Functional consequences for the enzyme ppt

... Molecu-lar cloning and cold-inducible gene expression of ferritinH subunit isoforms in rainbow trout cells. J Biol Chem271, 26908–26913.13 Tang SJ, Sun KH, Sun GH, Lin G, Lin WW & ChuangMJ ... affecting rates of transcription and ⁄ or transla-tion and ⁄ or mRNA and protein degradation. Thisrepresents a quantitative strategy to offset the lack orexcess of kinetic energy (as a measure of ... protein. 1v6a.pdb des-cribes secondary, tertiary and quaternary structures of LDH-A from the skeletal muscle of common carpincluding subunit and ligand interactions. Therefore,the structure of...

Tài liệu Báo cáo khoa học: Inhibitory effects of nontoxic protein volvatoxin A1 on pore-forming cardiotoxic protein volvatoxin A2 by interaction with amphipathic a-helix doc

... N-terminal and theC-terminal pair of a-helices of VVA1 can bind toVVA2 independently of each other and thus enable thedirect binding by one molecule of VVA1 of two mole-cules of VVA2. This ... oligomerization of VVA2 and thusits binding to simple lipid membranes (Fig. 1B, lanes 1 and 2). Therefore, this investigation of the structural and binding characteristics of VVA1 indicates thatVVA1 ... (B) Inhibitory effects of VVA1 on the binding of VVA2 to liposomes. Afterincubation of VVA1, VVA2 or the mixture of VVA2 and VVA1 at a molar ratio of 2 with liposomes (5 mM)at37°C for 30 min,...

Tài liệu Báo cáo khoa học: Functional effects of deleting the coiled-coil motif in Escherichia coli elongation factor Ts pptx

... rates in the presence of kirromycinThe effect of kirromycin on the growth of GRd.tsf wasinvestigated by measuring growth rates of cultures in LBcontaining various concentrations of kirromycin. ... nucleotideexchange in EF-Tu by increasing the dissociation of GDP and binding of GTP [26]. In addition, the binding of kirromycin and EF-Ts to EF-Tu are mutually exclusive [27].Alignment of several ... functional effects of deleting the motif in EF-Ts, both in vivo and in vitro. The motif was deleted in endogenous EF-Ts in E. coli strain UY211 by genereplacement, and the phenotype of the resulting...

Tài liệu Báo cáo Y học: Differential effects of arachidonoyl triﬂuoromethyl ketone on arachidonic acid release and lipid mediator biosynthesis by human neutrophils pot

... 1-acyl-linked phospholipids (mainlyPtdCho and PtdIns) was being remodeled into the ether-linked subclasses (1-alkyl-2-AA-GPC and 1-alk-1-enyl-2-AA-GPE).Examination of SA of products indicated ... synthetase, inhibition of CoA-IT results in anincrease in free AA and a corresponding build-up of AA in triacylglycerols that can be prevented by co-incubation of cells with CoA-synthetase inhibitors ... accompanied the incorporation of this AAinto 1-acyl-linked phospholipid subclasses (mainly in PtdCho/PtdIns). Conversely, the increase in the SA of ether-linked PtdEtn and PtdCho subclasses suggested...

Tài liệu Báo cáo khoa học: Proteomic analysis of dopamine and a-synuclein interplay in a cellular model of Parkinson’s disease pathogenesis docx

... dopamine treatment, overexpres-sion of a-synuclein signiﬁcantly affected the levels of four proteins [stathmin 1, glutathione S-transferase(GST)p, Ran 1 binding protein and C1q binding pro-tein], ... suggested to be linked toearly stages of PD pathogenesis [26]. Interestingly, in our model, dopamine induces an increase of the actinbundles regulator fascin 1 [27] and discordant changes of two calcium-dependent, ... of ββ-Actinα-SynucleinFig. 1. Relative expression of a-synuclein in b-gal and a-syn cells in response to dopamine (DA) treatment. Results are indicated as thefold of induction relative to expression observed in...

Tài liệu Báo cáo khoa học: Molecular defect of isovaleryl-CoA dehydrogenase in the skunk mutant of silkworm, Bombyx mori ppt

... leucine degradation and thesku mutant accumulates branched-chain amino acids in haemolymph, thismutant may be useful in the investigation of unique branched-chain aminoacid catabolism in insects.AbbreviationsACAD, ... that of the enzyme-containing solution and was expressed as nmols of 2,6-dichloroindophenol reduced per mg of protein permin. The extinction coefﬁcient of 2,6-dichloroindophenol(21 000 MÆcm)1) ... size and expression levels(Fig. 2A). Determination of full-length cDNAsequences using the RACE method in both strainsrevealed the presence of a single point mutation in 1337 nucleotides of BmIVD....

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