Tài liệu Báo cáo khoa học: Identification of two late acyltransferase genes responsible for lipid A biosynthesis in Moraxella catarrhalis doc

... Catlin BW (1990) Branhamella catarrhalis: an organismgaining respect as a pathogen. Clin Microbiol Rev 3,293–320.2 Karalus R & Campagnari A (2000) Moraxella catarrh-alis: a review of an ... AAG CCG ATG ACA CCA ATT (asd sense) This studyasd2 GCA GGT TCA TAG TGC ATG (asd antisense) This studyKan RP GGT GCG ACA ATC TAT CGA (kanamycin sense) [19]Kan FP CTC ATC GAG CAT CAA ATG (kanamycin ... is a biological component of the lipo-oligosaccharide of a humanpathogen, Moraxella catarrhalis. No other acyltransferases except for UDP-GlcNAc acyltransferase, responsible for lipid A biosynthesis...

... C), for pGEX–EWS; EAD forward d(CGGAAT TCA TGG CGT CCA CGG ATT ACA G) and EADreverse d(CGC TCG AGT CAT CCG GAA AAT CCTCCA GAC T), for pGEX–EAD; RGG1 forward d(CGGAAT TCC CAG GAG AGA ACC GGA ... protein as a G-quadruplex DNA- and RNA-binding proteinKentaro Takahama1,*, Katsuhito Kino2,*, Shigeki Arai3, Riki Kurokawa3and Takanori Oyoshi11 Department of Chemistry, Faculty of Science, ... Proteins 61,164–175.48 Raman B, Guarnaccia C, Nadassy K, Zakhariev S,Pintar A, Zanuttin F, Frigyes D, Acatrinei C, Vindigni A, Pongor G et al. (2001) Nx-arginine dimethylationmodulates the interaction...

... AATGCTGGCTCTCCCTCGATGCTTGGCTACTGGACCATCAAHYPK GGAAATGGAAATAACAAGACAAATAGCGCGCAACTAATGCTTCCACAAHSP70 TGACCAAGGCAACAGAACCAAATCAGACGGCCGGTATGTGHeat shock 70 kDa protein 1 2A CGAAAAAGGACAGCAGTTGAAACTCATCCTCCACCGGATTGTHSP23 ... in B cells, kinasecomplex-associated proteinAAAGCAGAGCAGAAAAAGTGGAAGGACAATGCCGCGATCAGNon-selenium glutathione peroxidase CAATGAACAAAAAAGTCGCAACAGGGATGGAGGGTAAGACCATACAGlutamine synthetase ... CGTCCGATTTCTTCTCGTGTTTACCAGAAGACATTACAGTGAAAATTGAChaperonin-containing TCP1, subunit 7, isoform b, isoform 1 GGGAACCAGCAGTCGTCAAACGTCCACTGAGAGGATGAGACAInhibitor of kappa light polypeptide enhancer...

... M, Hasegawa K, HoritaC & Akera S (1999) A new matrix protein familyrelated to the nacreous layer formation of Pinctadafucata. FEBS Lett 462, 225–229.5 Kono M, Hayashi N & Samata T ... 126,511–520.27 Tohse H, Murayama E, Ohira T, Takagi Y & Nagasa-wa H (2006) Localization and diurnal variations of car-bonic anhydrase mRNA expression in the inner ear of the rainbow trout Oncorhynchus ... to have a molecular mass of 64 kDa, and to contain two tandem repeats and a Glu-rich region. The structure of the proteinand that of its DNA are similar to those of starmaker, a protein involvedin...

... cerevisiaestrain GIL77 Two oligo DNAs (5¢-CTTCGTCGACAAGATGTGGAGGTTGAAGATA-3¢ and 5¢-GTCCGCTAGCTCAAGGCAAAGGAACTCTTCT-3¢), corresponding to the N- andC-terminal sequences of b-amyrin synthase from ... Cloning and characterization of a cDNA encod-ing b-amyrin synthase involved in glycyrrhizin andsoyasaponin biosynthesis in licorice. Biol Pharm Bull 24,912–916.16 Hayashi H, Huang P, Inoue ... CYP93E1 for b-amy-rin and sophoradiol suggests that the biosynthesis of soyasaponin might form a metabolic grid, viaFig. 6. GC-MS analysis of in vitro reaction products with b-amyrin as a substrate....

... in the absence of the STAT2 DNAbinding domain as revealed by real-time PCRTo more quantitatively examine the expression of IFN-regulated genes in the absence of the STAT2 DNAbinding domain, ... on a functional STAT2 DNA bindingdomain do not appear to play an important role in mediating the transcription of these genes. The c-fos gene was examined in this system as anexample of a GAS-driven ... IFN-inducible transcriptional activation in the absence of the STAT2 DNA binding domain as determined by Affymetrix DNA microarrayanalysis. Total mRNA samples from U 6A- 2, U 6A- 2VV-II and U 6A cells...

... 5¢-GGAUCAAAGAGAACACUGGGGUAUA-AG-3¢;and #1513 sense, 5¢-AGUUCACGUGCAAGAACAAGUUCUG-AG-3¢. The forward sequence of the scrambledRNA was 5¢-GAUCCAAGUAAUACAGAGAUGGGAGAG-3¢. OVISE cells were plated the day before ... The main fraction contained a 75 kDamatriptase as a major component and a few contamin-ating proteins as analyzed by SDS ⁄ PAGE.RNAi experiments with OVISE cellsMatriptase siRNAs and a scrambled ... RNA as a controlwere designed and synthesized at iGENE (Tsukuba, Japan).The forward sequences of the siRNAs were: #973, sense5¢-UCAUCACACUGAUAACCAACACUGA-AG-3¢;#2578,sense 5¢-GGAUCAAAGAGAACACUGGGGUAUA-AG-3¢;and...

... ACCACTTTGTACAAGAAAGCTGGGTyef1hisfCAATAAATCTGCTTACGTGACATTTTTTACTAAAAGAGAATATGCGTACGCTGCAGGTCGACyef1hisrGAACCCTTGACTACGGAAACGCAGGATGTGGGAAATCGTTAATCGATGAATTCGAGCTCGpos5hisfCATAAATAAAAGGATAAAAAGGTTAAGGATACTGATTAAAATGCGTACGCTGCAGGTCGACpos5hisrCTTAGAGAATCTCATTGAATCTTTGCATTCAGAGCGTTTAATCGATGAATTCGAGCTCGpos5leu21.6fCATAAATAAAAGGATAAAAAGGTTAAGGATACTGATTAAAATGCCAATTCTGTGTTTCCCGGAAATGpos5leu21.6rCTTAGAGAATCTCATTGAATCTTTGCATTCAGAGCGTTTAGTAAAGTTCGTTTGCCGATACATGyef1up0.5kbCGTTATGAAAATCACTATTATCCCCyef1-HindIII ... cerevisiae is underlined.Primer Oligonucleotide sequencesyef1-attB1FSD AAAAAGCAGGCTCCGAAGGAGATATAAAAATGAAAACTGATAGATTACTGyef1-attB2R AGAAAGCTGGGTGGATTGCAAAATGAGCCTGACattB1 ACAAGTTTGTACAAAAAAGCAGGCTattB2 ... ACAAGTTTGTACAAAAAAGCAGGCTattB2 ACCACTTTGTACAAGAAAGCTGGGTyef1hisfCAATAAATCTGCTTACGTGACATTTTTTACTAAAAGAGAATATGCGTACGCTGCAGGTCGACyef1hisrGAACCCTTGACTACGGAAACGCAGGATGTGGGAAATCGTTAATCGATGAATTCGAGCTCGpos5hisfCATAAATAAAAGGATAAAAAGGTTAAGGATACTGATTAAAATGCGTACGCTGCAGGTCGACpos5hisrCTTAGAGAATCTCATTGAATCTTTGCATTCAGAGCGTTTAATCGATGAATTCGAGCTCGpos5leu21.6fCATAAATAAAAGGATAAAAAGGTTAAGGATACTGATTAAAATGCCAATTCTGTGTTTCCCGGAAATGpos5leu21.6rCTTAGAGAATCTCATTGAATCTTTGCATTCAGAGCGTTTAGTAAAGTTCGTTTGCCGATACATGyef1up0.5kbCGTTATGAAAATCACTATTATCCCCyef1-HindIII...

... therefore provide additional critical infor-mation over that obtained from X-ray data alone. Theknowledge gained from these mutagenesis data will bevaluable in directing the design of modulators ... side chain of Arg273 is therefore crit-ical for binding of the substrate. Maintaining the posit-ive charge at this position (R273K) is not sufficient for docking of the peptide into the ACE2 active ... The zinc prote-ase domain of both tACE and ACE2 is divided into two subdomains[20]. Subdomain I contains the zinc ion and the N-terminus. TheC-terminus is found in subdomain II.tACE ACE2Subdomain...

... auto-radiographed. In the UV cross-linking assay, the samples were incuba-ted at room temperature, as described above for the EMSAassay, and then irradiated at 302 nm for 10 min using a transilluminator ... different isoforms of eEF 1A in the nuclear fraction of human T-lymphoblastic cancer cell line specifically bindingto aptameric cytotoxic GT oligomersBarbara Dapas1, Gianluca Tell2, Andrea Scaloni3, ... techniqueand a- cyano-4-hydroxycinnamic as matrix, and analysed byusing a Voyager-DE PRO mass spectrometer (AppliedBiosystems, Framingham, MA, USA). Internal-mass calib-ration was performed with...