Tài liệu Báo cáo khoa học: Expression of poly(A)-binding protein is upregulated during recovery from heat shock in HeLa cells doc

... of PABP1 during the phase of recovery from heat shock may be neces-sary to meet the cellular demand for protein synthesisfor complete recovery from stress.ResultsPABP1 expression during recovery ... TOP cis-element of PABP1mRNA is involved in the upregulation of PABP1mRNA translation during recovery from heat shock. Previous studies have shown that translation of TOP-containing mRNAs is ... heat shock recovery S. Ma et al.570 FEBS Journal 276 (2009) 552–570 ª 2008 The Authors Journal compilation ª 2008 FEBS Expression of poly(A)-binding protein is upregulated during recovery from heat...

Tài liệu Báo cáo khoa học: Comparison of a coq7 deletion mutant with other respiration-defective mutants in ﬁssion yeast doc

... Induction of ctt1, encoding catalase, and apt1, encodinga 25 kDa protein, but not that of gpx1, encoding glutathione peroxidase,was indistinguishable in four ubiquinone-deficient mutants, indicating ... phenotypes were indis-tinguishable from those of other ubiquinone-deficientSc. pombe mutants, which argues against a possiblerole for demethoxyubiquinone in respiration.ResultsCloning of coq7 and ... Whereas induction of ctt1+and apt1 occurred in all ubiquinone-deficient strains, induction of gpx1+was not observed in any of the tested strains (Fig. 6).However, in the wild-type strain treated...

Báo cáo khoa học: Inhibition of Hsp90 function delays and impairs recovery from heat shock ppt

... heat shock and recovery. In keeping with theobservation that geldanamycin did not inhibit protein synthesis per se, no difference in protein synthesis rate during heat shock or early in the recovery ... B (1989)Possible involvement of the 90-kDa heat shock protein in the regulation of protein synthesis. J Biol Chem 264,6239–6244.R. F. Duncan Ansamycins inhibit heat shock recovery FEBS Journal ... areseverely inhibited, presumably to minimize the accu-mulation of deleterious protein forms. Second, there is a massive, rapid synthesis of a small group of proteinstermed the heat shock proteins...

Tài liệu Báo cáo khoa học: Expression of an a-1,3-glucanase during mycoparasitic interaction of Trichoderma asperellum docx

... simulated antagonism. The expression of agn13.2 during in vivo assays against the strawberrypathogen Botrytis cinerea strongly supports the role of AGN13.2 in the antagonistic action of T. asperellum.ResultsBiochemical ... with this idea, it is worth mention-ing that both strains display clear distinctive host rangeand antagonistic abilities in controlled assays (unpub-lished results).Gene expression in the ... considered in each determination.Thermal stability of the enzyme was determined incubatingthe puriﬁed protein at temperatures from 25 to 70 °Cin50 mm sodium acetate for 30 min and then measuring...

Tài liệu Báo cáo khoa học: Expression of two [Fe]-hydrogenases in Chlamydomonas reinhardtii under anaerobic conditions doc

... metabolism. This hypothesis will be investigated in thefuture.The HydA2 protein is expressed during anaerobicinduction In order to determine whether HydA2 is an expressed protein, HydA2-speciﬁc oligo-peptide ... recognized a protein of about 49 kDa in extracts from anaerobically inducedC. reinhardtii cells, strongly suggesting that HydA2 encodesfor an expressed protein. Homology-based 3D modeling of the ... differences in the regulation of expression of the two genes.Gene expression during anaerobic inductionThe coexpression of the HydA1 and HydA2 transcripts wasstudied by Northern blot analysis. In Fig....

Tài liệu Báo cáo khoa học: Role of the cag-pathogenicity island encoded type IV secretion system in Helicobacter pylori pathogenesis pptx

... b-catenin distribution,making them unsuitable for the investigation of b-cate-nin signalling [29]. In support of this aspect, usingMadin–Darby canine kidney cells that expressE-cadherin and ... have indi-cated that the CagAPY-induced cell elongation pheno-type also involves tyrosine dephosphorylation of cortactin, vinculin and ezrin, which are three well-known actin-binding proteins ... phosphatasesinvolved in this scenario, however, remain unknownand do not require Shp2. Instead, CagAPYcan inhibitSrc activity both by direct interaction of both proteinsor by binding of CagAPYto...

Tài liệu Báo cáo khoa học: Regulators of G-protein signalling are modulated by bacterial lipopeptides and lipopolysaccharide pptx

... proteins, and to the expression of pro- and in ammatory cytokines. There is evidence that the heteromeric guanine nucleotide-binding regulatory protein (G protein) is also involved in TLR4 activation. ... little is known about the involve-ment of RGS2 proteins in the context of in ammation.Under all RGS proteins, RGS2 contains a uniquefunction, because it is the only RGS protein that doesnot interact ... of regulator of G protein signaling proteins in dendritic cells: implications for G protein- coupled receptor signal-ing. J Immunol 172, 5175–5184.4 Hoebe...

Tài liệu Báo cáo khoa học: Modulation of sterol homeostasis by the Cdc42p effectors Cla4p and Ste20p in the yeast Saccharomyces cerevisiae pptx

... functional link behind this ﬁnding is not clear.Interestingly, Ste20p and Cla4p also down-regulatesterol uptake by inhibiting the expression of genesinvolved in this process (Lin and Ho¨fken, ... monitor protein protein interac-tions, and were grown for 2 days at 30 ° C. Protein analysis Protein concentration was determined, as described previ-ously [66], using BSA as a standard. Proteins ... sterols in mating [28,29] and it has been suggested that sterolbiosynthesis may increase during formation of a matingprojection [28]. Furthermore, homologues of oxysterol-binding proteins, a...

Tài liệu Báo cáo khoa học: Characterization of chitinase-like proteins (Cg-Clp1 and Cg-Clp2) involved in immune defence of the mollusc Crassostrea gigas docx

... gonadic tubules and the rebuilding of storage tissues [22]. The expression of Cg-Clp2 during this particular period is somewhat reminiscent of theﬁnding that certain mammalian CLPs such as CLP-1and ... FEBS from the oyster Crassostrea gigas [5]. Interestingly, this protein, named C. gigas chitinase-like protein 1 (Cg-Clp1) was found to be involved in the control of growth and remodelling processes ... mainly expressed during larvalmetamorphosis, in the mantle edge and the digestivegland. During the reproductive cycle, expression washigh in gonads during the postspawning period butnot in...

Tài liệu Báo cáo khoa học: Role of receptor-mediated endocytosis, endosomal acidiﬁcation and cathepsin D in cholera toxin cytotoxicity pdf

... parenchyma. Following administration of a saturating dose of cholera toxin to rats, rapid endocytosis of both cholera toxin subunits wasobserved, coincident with massive internalization of both the ... prepared from CT and CT-B-injected rats (Fig. 3A). An increase in ARF content was observed in endosomal fractionsisolated 5 min postinjection of CT-B, and this increasewas maintained for up to 60 min ... trans-Golgi network. In theER, the disulﬁde bond linking CT-A1 to CT-A2 ⁄CT-B5 is reduced by protein disulﬁde isomerase, andCT-A1 is then translocated to the cytosol in a processinvolving ER-associated...

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