Tài liệu Báo cáo khoa học: Molecular cloning and characterization of soybean protein disulfide isomerase family proteins with nonclassic active center motifs pdf
... family proteins
FEBS Journal 276 (2009) 4130–4141 ª 2009 The Authors Journal compilation ª 2009 FEBS 4141
Molecular cloning and characterization of soybean
protein disulfide isomerase family proteins ... (2008) A novel
plant protein disulfide isomerase family homologous to
animal P5 – molecular cloning and characterization as a
functional protein for...
... expression of T-bet has been
seen within a wide variety of tissues and cells in the
Ginbuna crucian carp [27] and, in mammals, in the
lung tissue of mouse and spleen and thymus of human
and mouse ... the expression
of these genes within humans and mice, of T-bet
within Ginbuna crucian carp [27] and STAT6 in
mandarin fish [28] have shown similar widespread
expression. I...
... spe-
cificity [31]. The L3 loop of SmSmad1B groups this
protein with other BMP-related R-Smads. The presence
of a histidine and an aspartate at positions 340 and 343
within the L3 loop of SmSmad1B (Fig. 1A) ... schisto-
somes, and also confirm the similarities in the protein
interaction properties of SmSmad1 and SmSmad1B as
described above. However, in the presence of Sm...
... dithiothreitol
(DTT) and 20% (v ⁄ v) glycerol, and 20% saturation of
(NH
4
)
2
SO
4
. The enzyme was eluted with a linear gradient
of 30–0% saturation of (NH
4
)
2
SO
4
in buffer A. Active
fractions ... (5734 Da), cyto-
chrome c (12361 Da), myoglobin (16952 and 8476 Da) and
ubiquitin (8566 Da; all Sigma) as standard proteins.
Western blotting
Proteins from SDS ⁄ PAGE wer...
... intense
band of 61 kDa (NrfA) and a band of weak intensity of
19 kDa (NrfH), confirming its hetero-oligomeric nature
(Fig. 1, lane 1).
However, in the absence of boiling (Fig. 1A, lanes 2 and
4) high molecular ... structural proteins during the
assembly of the head of bacteriophage T4. Nature 227, 680–685.
28. Blum, H., Beier, H. & Gross, H.J. (1987) Improved silver sta...
... erythro-
cytes.
De-O-acetylated preparation of glycoproteins. De-O-
acetylation of glycoproteins was performed following the
procedures of Sarris and Palade [30] and Schauer [6]. A
solution of 750 lL of glycoprotein (5 mgÆmL
)1
) ... inhibition (HAI) assays with a
variety of sialoglycoproteins (Table 5) showed BSM as the
most potent inhibitor of the purified lectin with a...
... sulphate-reducing archaeon Archaeoglobus f ulgi dus:
cloning and characterization of the genes and c omparison of t he
enzyme with other i ron-sulphu r flavoproteins. Microbiology 140,
1273–1284.
36. Hipp,W.M.,Pott,A.S.,Thumschmitz,N.,Faath,I.,Dahl,C.&
Tru
¨
per, ... is
typical of integral membrane proteins. The polypeptide with an
apparent molecular mass of 53 kDa a...
... (2008) A novel
plant protein disulfide isomerase family homologous to
animal P5: molecular cloning and characterization as a
functional protein for folding of soybean seed storage
proteins. FEBS J ... substrate proteins. Mapping of associa-
tion sites of chaperones and PDI family proteins along
individual substrate polypeptides will be necessary for
clarificati...
... identification and characterization of other mem-
bers of the CYP719 family, especially in evolutionally
older plant species, should provide clues to better
understand the diversification of the CYP719 family
and ... parameters of
CYP719A3, the amount of nandinine produced was estima-
ted by using the calibration curve of (S)-scoulerine (pmol
versus peak area) and the abs...
... mixture was extracted once with phe-
nol ⁄ chloroform and once with chloroform, and RNA was
precipitated with 2-propanol and dissolved in RNase-free
water. Single-stranded RNAs were allowed to ... roots of the thoracic ganglia and
in the subesophagic ganglion of Homarus americanus
[15,16]. It is also detected in the retina of the crayfish
Procambarus clarkii [17].
The clon...