... control was maintained in a standard incubator with 21% O2. The normoxia data are the same as shown in Fig. 1 and are included here for comparison. Expression of miR-2 7a and miR-27b at the indicated ... were treated as described in (A) . Total RNA wasprepared at the indicated times and subjected to quantitative real-time PCR analysis. The data shown are mean value ± standard errors of the mean from ... Dharmacon, Lafayette, CO, USA). Usingquantitative real-time PCR analysis, we found a > 60-fold increase in mature miR-2 7a and miR-27b in the transfected preadipocytes. As shown in Fig. 2A, miR-2 7a, ...