Tài liệu Báo cáo khoa học: Molecular characterization of Arabidopsis thaliana PUF proteins – binding speciﬁcity and target candidates doc

... greatnumber of Arabidopsis transcripts are potential targetsfor regulation by the PUF family of proteins. Theresults obtained reveal a molecular conservation of PUF proteins in Arabidopsis thaliana and ... the binding speciﬁcity of the subset of groupI APUM proteins, showing that A. thaliana has at leastsix PUF proteins with conserved RNA -binding and similar speciﬁcity. The group I APUM proteins ... alignment of the APUM proteins with the PUF domains most similar to Drosophila PUF domain. (A) APUM proteins of group I. (B) APUM proteins of group II.C. W. Francischini and R. B. Quaggio PUF proteins...

Tài liệu Báo cáo khoa học: Biochemical characterization of Bacillus subtilis type II isopentenyl diphosphate isomerase, and phylogenetic distribution of isoprenoid biosynthesis pathways doc

... c-proteobacteria A. vinelandii and P. luminescens and to Idi-1 protein of the BacteroidC. hutchinsonii (expect values 3e-22, 5e-19 and 8e-20,respectively).Paralogs of Idi-2 proteins Database searches ... protein is clearly a member of a superfamily of (S)-a-hydroxyacid dehydrogenases, and the coenzyme pattern of Idi-2 protein, where the roles of FMN and of NADPHrequired under aerobic conditions ... (1996) SEAVIEW and PHYLO_WIN: two graphic tools for sequence alignment and molecular phylogeny. Comput. Applic. Biosci. 12, 54 3–5 48.39. Dayhoﬀ, M.O. (1979) Atlas of Protein Sequence and StructureVol5...

Tài liệu Báo cáo khoa học: Molecular characterization, phylogenetic relationships, and developmental expression patterns of prion genes in zebraﬁsh (Danio rerio) doc

... residuesinstead of 15 residues, including strand S2, betweenhelices H1 and H2, and 84 residues instead of 10 resi-dues between helices H2 and H3 (Fig. 2). Molecular characterization of zebraﬁsh ... Transcripts of this gene and PrPCare present ina large variety of adult peripheral tissues [1 8–2 0].In contrast, there is a paucity of data on the spatio-temporal expression of prion proteins and ... the time and sites of PrP1 and PrP2 action(s) during nervoussystem morphogenesis and also suggest that PrP2 hasa pleiotropic role in the course of embryogenesis. Ahigh expression of zebraﬁsh...

Tài liệu Báo cáo khoa học: Molecular characterization and allergenic activity of Lyc e 2 (b-fructofuranosidase), a glycosylated allergen of tomato pdf

... Manb 1–4 GlcNAcb 1–4 (Fuca 1–3 )GlcNAc) 5.3MMX (Mana 1–6 (Mana 1–3 )(Xylb 1–2 )Manb 1–4 GlcNAcb 1–4 GlcNAc) 8.2MMXF3(Mana 1–6 (Mana 1–3 )(Xylb 1–2 ) Manb 1–4 GlcNAcb 1–4 (Fuca 1–3 )GlcNAc) 83.6GnMXF3(GlcNAcb 1–2 Mana 1–6 (GlcNAcb 1–2 Mana 1–3 )(Xylb 1–2 )Manb 1–4 GlcNAcb 1–4 (Fuca 1–3 )GlcNAc) ... 83.6GnMXF3(GlcNAcb 1–2 Mana 1–6 (GlcNAcb 1–2 Mana 1–3 )(Xylb 1–2 )Manb 1–4 GlcNAcb 1–4 (Fuca 1–3 )GlcNAc) 2.3GnGnMXF3(GlcNAcb 1–2 Mana 1–6 (Mana 1–3 )(Xylb 1–2 )Manb 1–4 GlcNAcb 1–4 (Fuca 1–3 )GlcNAc) 0.6Fig. 5. Immunoblot inhibition of IgE reactivity of a serum ... elution fractions 3 0–3 3 Lyc e 2 with a molecular mass of 50 kDa was eluted, fractions 3 4–3 7 contained a band of 36 kDa and the fractions 3 8–4 1 a protein with a molecular mass of about 20 kDa (Fig....

Tài liệu Báo cáo khoa học: Molecular basis of glyphosate resistance – different approaches through protein engineering doc

... some of the key ﬁndings that have led to our current under-standing of the molecular mode of action of glypho-sate and the molecular basis for glyphosate resistance.Structure and function of ... (Arg21, Arg111, and His138) and with thecarboxylate end (Arg21 and Arg73) of GriP. Of partic-ular note is a short, 2.46-A˚hydrogen bond between N-e of His138 and a phosphate oxygen of GriP.Alanine ... uponsubstrate binding and catalysis [16,19,20].Discovery and engineering of glyphosate-resistantEPSPSThe extraordinary success of glyphosate is attributable,in large part, to the high speciﬁcity of...

Tài liệu Báo cáo khoa học: Molecular defect of isovaleryl-CoA dehydrogenase in the skunk mutant of silkworm, Bombyx mori ppt

... bacte-rial and baculovirus chitinases. Insect Biochem Mol Biol33, 74 9–7 59.30 Laemmli UK (1970) Cleavage of structural proteins during assembly of head of bacteriophage-T4. Nature227, 68 0–6 85.31 ... Shimizu4,5,Susumu Katsuma1 and Toru Shimada1,41 Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, University of Tokyo, Japan2 Institute of Genetic Resources, ... Unit, Graduate School of Agricultural and Life Sciences, University of Tokyo, Japan5 Department of Biotechnology, Graduate School of Agricultural and Life Sciences, University of Tokyo, JapanIntroductionIsovaleryl-CoA...

Tài liệu Báo cáo khoa học: Functional characterization of an orphan cupin protein from Burkholderia xenovorans reveals a mononuclear nonheme Fe2+-dependent oxygenase that cleaves b-diketones ppt

... ligand binding, and the role of storage proteins, the cupins constitute one of the mostdiverse superfamilies of proteins known. They havebeen described from all three domains of life [1,2], and usually ... an average distance of 2.03 A˚for Fe–N(His)was inferred [38], and a target distance of between 1.93 and 2.13 A˚for Fe–O was obtained [39]. Consideringthe proposed mode of substrate coordination ... ﬁngerprints and prediction of oligomerization and metal cofactor fromsequence. Proteins 70 , 156 4–1 577.9 Dai Y, Wensink PC & Abeles RH (1999) One protein,two enzymes. J Biol Chem 274, 119 3–1 195.10...

Tài liệu Báo cáo khoa học: Molecular determinants of ligand speciﬁcity in family 11 carbohydrate binding modules – an NMR, X-ray crystallography and computational chemistry approach doc

... for screening and identifying ligand binding toprotein receptors. Angewandte Chemie-International Edi-tion 42, 86 4–8 90.16 Mayer M & Meyer B (1999) Characterization of ligand binding by saturation ... is a common binding site at the Ct CBM11ABCFig. 4. Representation of the conformations of the 3D structure of binding of the differ-ent ligands obtained by docking. (A) a- (red) and b-cellobiose ... system with constraints – molecular- dynamics of N-alkanes. J Comput Phys 23, 32 7–3 41.40 Pastor IWR, Brooks BR & Szabo AJ (1998) An analy-sis of the accuracy of Langevin and molecular- dynamicsalgorithms....

Tài liệu Báo cáo khoa học: Kinetic characterization of the ﬁrst step of the ribozyme-catalyzed trans excision-splicing reaction docx

... effectEE*Sk2= 4.1 min –1 k1= 1 x 107 M –1 ·min –1 k –1 = 0.9 min –1 k –3 = 0.09 min –1 k3= 3.5 x 103 M –1 ·min –1 E + PKdP= 69 nMKdS= 90 nMkc= 5.7 min –1 EPFig. 2. Kinetic scheme for ... recognition. 3. Kinetics of the helix–coiltransition of the oligoribouridylic–oligoriboadenylicacid system and of oligoriboadenylic acid alone atacidic pH. J Mol Biol 62, 36 1–3 81.39 Nelson JW & ... 836 2–8 366.22 Herschlag D, Eckstein F & Cech TR (1993) Contribu-tions of 2¢-hydroxyl groups of the RNA substrate to binding and catalysis by the Tetrahymena ribozyme. Anenergetic picture of...

Tài liệu Báo cáo khoa học: Molecular aspects of rheumatoid arthritis: chemokines in the joints of patients pdf

... joints of the hands and feet. Theinflammatory process is characterized by infiltration of inflammatory cellsinto the joints, leading to proliferation of synoviocytes and destruction of cartilage and ... against the development of RA [61].The gene copy number variations of CCL3-like 1(CCL3L1), a nonallelic isoform of CCL3 encoded bydifferent genes and a potent ligand for CCR1 and CCR5, influenced ... source of MCP-1 ⁄CCL2 production [19]. The levels of MCP-1 ⁄ CCL2correlate significantly with the levels of IL-1b, IL-6 and IL-8 ⁄ CXCL8 in culture supernatants of synoviumfrom RA patients, and...

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