... Biochemistry,Biocenter, Johann Wolfgang Goethe-University, Max-von-Laue-Strasse 9,D-60348 Frankfurt am Main, GermanyFax: +49 (0) 69 798 29495Tel: +49 (0) 69 798 29475E-mail: tampe@em.uni-frankfurt.deWebsite: ... ER-resident transporter are oriented tothe cytosol. The localization is not influenced by addi-tional C- or N-terminal His-tags either for full-lengthor leaderless MDL1.To exclude that full-length ... particular,arginine can be enriched in position -2 , -3 , -1 0, and -1 1relative to the cleavage site. The leader sequence inter-acts with the TOM receptor, responsible for the trans-location of the preproteins...