Tài liệu Báo cáo khoa học: Bacitracin is not a speciﬁc inhibitor of protein disulﬁde isomerase pptx

... that bacitracin should not be regarded as a specific inhibitor of PDI.Results Bacitracin does not inhibit the catalysis of disulfide bond formation and isomerization by PDIPDI is a catalyst of ... Bacitracin is not a specific inhibitor of protein disulfide isomerase Anna-Riikka Karala and Lloyd W. RuddockBiocenter Oulu and Department of Biochemistry, University of Oulu, FinlandIntroduction Protein ... the effect of bacitracin in a variety of in vitro assays for PDI activity. On the basis of the BPTI refolding assay and peptide oxidationassay 1 mm bacitracin does not have a significanteffect...

Tài liệu Báo cáo khoa học: Mixed lineage leukemia: a structure–function perspective of the MLL1 protein ppt

... CREB-bindingdomain of CBP [31]. The KIX domain of CBP is a structural platform that is capable of binding severaldifferent families of transcriptional activators [30], andevidence indicates that the ... lysinemethyltransferases. J Biol Chem 280, 5563–5570.53 Qian C, Wang X, Manzur K, Sachchidanand, Farooq A, Zeng L, Wang R & Zhou MM (2006) Structuralinsights of the speciﬁcity and catalysis of a viralhistone ... protein and its homolog p300 are generaltranscriptional co-activators that contain histone andtranscription factor acetylation activities [30]. In addi-tion, CBP contains a number of protein- bindingdomains...

Tài liệu Báo cáo khoa học: Multiple promoters regulate tissue-speciﬁc alternative splicing of the human kallikrein gene, KLK11⁄hippostasin ppt

... 5¢-CTGCCGTCTCCGCCGCCACT-3¢; FU, 5¢-TCAAGCCCCGCTACATAGTT-3¢; and R3, 5¢-AGGAACCAAACACCAAGTGG-3¢(Fig. 2A) .Luciferase promoter assayHuman genomic DNA was isolated from two volunteers(both male, Japanese, aged ... tissue -speciﬁc use of each promoter. We show the translation initiation site of isoform 2 and different intracellular distribution of isoforms 1 and 2, and discuss the regulatory mechan-ism of ... raised against KLK11 was the same sizeas that of the translational product by isoform 2mRNA (Fig. 4B). The product by isoform 1 mRNAwas smaller than the isoform 2 product. The multiplebands...

Tài liệu Báo cáo khoa học: "Topological Dependency Trees: A Constraint-Based Account of Linear Precedence" ppt

... an ‘oblig-atory auxiliary flip’.(18) (dass)(that)MariaMariaeinen a Mannmanhathasliebenlovekönnencan(that) Maria was able to love a man(19) (dass) Maria hat einen Mann lieben ... behavior is called ‘optional auxiliary flip’.(11) (dass)(that)MariaMariaeinen a Mannmanliebenlovekönnencanwirdwill(that) Maria will be able to love a man(12) (dass) Maria einen Mann wird ... andNakazawa, 1994), the verbal complement of anauxiliary verb, such as haben or werden, followsrather than precedes its head. Only a certain class of bare infinitive verbs can land in extraposed...

Tài liệu Báo cáo khoa học: NirF is a periplasmic protein that binds d1 heme as part of its essential role in d1 heme biogenesis pdf

... in a mutant thatlacks NirF; this too is not trivial as the DnirF straindoes not accumulate readily detectable amounts of anintermediate of d1synthesis.Experimental proceduresDNA manipulationsDNA ... biogenesis suggests that the activity of NirF cannot be similar to that of Met8P activitywhere a comparable mutation is inhibitory. A puzzle is that some NirF sequences, notably for two strains of ... 252.221.81.41.21.610.80.60.40.20 A 600 A 600 A 600Time (h)Fig. 5. His41 is essential for Paracoc-cus pantotrophus NirF, but Asp129 is dis-pensable. Growth plots and time courses of nitrite appearance and disappearance...

Tài liệu Báo cáo khoa học: It is all about resolution Meeting report based upon presentations at the 10th International Global BioMillennium 2006 symposium on molecular cell biology (Tbilisi, Georgia) docx

... that differ substantially in theirbiochemical properties and intracellular localization.DAP-kinases (DAPk), Ca2+⁄ calmodulin-regulatedand Ser ⁄Thr kinases, activate signaling pathways thatlead ... AaronCiechanover (Haifa, Israel), who argued that theubiquitin proteolytic system covers the pathway forelucidating the basic mechanisms that are pivotal fordrug targeting. Degradation of cellular proteins ... platforms. Appliedto six publicly available cancer microarray gene expres-sion datasets from three pairs of studies, this approachwas used for examining breast cancer, prostate cancerand acute...

Tài liệu Báo cáo khoa học: P25a ⁄ TPPP expression increases plasma membrane presentation of the dopamine transporter and enhances cellular sensitivity to dopamine toxicity pptx

... their analysis by SDS ⁄ PAGE.Subcellular fractionation of porcine striatal braintissuePorcine brain cut in half in the saggital plane was obtainedfresh from a local abattoir and immediately ... of DA is thought to be maintained by a regulatedbalance between the functional levels of plasma mem-brane DAT and vesicular VMAT-2.The protein a- synuclein (a- syn) is known to play a central ... DAT and p2 5a in subcellular fractions of porcine striatal tissue and localization in cells. (A) Isolated porcinestriatal tissue (nucleus caudatus) was fractionated as described inExperimental...

Tài liệu Báo cáo khoa học: Angiopoietin-like proteins: emerging targets for treatment of obesity and related metabolic diseases pptx

... Oike Y, Yasunaga K, Hamada K, Miyata K,Matsumoto S, Sugano S, Tanihara H, Masuho Y &Suda T (2003) Inhibition of angiogenesis and vascularleakiness by angiopoietin-related protein 4. Cancer ... Y, Akao M, Yasunaga K, Yamauchi T, MorisadaT, Ito Y, Urano T, Kimura Y, Kubota Y, Maekawa Het al. (2005) Angiopoietin-related growth factor antago-nizes obesity and insulin resistance. Nat ... reduction of visceral fatin obese diabetic patients treated with pioglitazone, a PPARc agonist with unique antidiabetic activity thatdecreases visceral fat, suppresses inﬂammation andameliorates...

Tài liệu Báo cáo khoa học: Mixed lineage leukemia: histone H3 lysine 4 methyltransferases from yeast to human pptx

... lysine of histone H3 is post-translationally modified by a methylgroup via the action of histone methyltransferase, and such a covalentmodification is associated with transcriptionally active and ... therapy combiningrelated proteins ⁄ pathways is a promising alternative.Thus, combinatorial therapy targeting HMTs, demeth-ylases, histone deacetylases, DNA methyltransferasesand others may ... efficiently in treating diseasescaused by epigenetic misregulations. In fact, a combi-natorial therapy targeting histone deacetylases andDNA methyltransferases has been shown to have a synergistic role...

Tài liệu Báo cáo khoa học: Neuronal growth-inhibitory factor (metallothionein-3): evaluation of the biological function of growth-inhibitory factor in the injured and neurodegenerative brain pdf

... M, Sogawa N, Miyazaki I,Nakanishi T, Furuta H & Ogawa N (2001) Localiza-tion, regulation, and function of metallothionein-III ⁄growth inhibitory factor in the brain. Acta MedOkayama 55, ... role of GIFin neuroﬁbrillary changes associated with AD.Potential role of metal-binding/exchangeproperties of GIF in ADOne of the primary pathological hallmarks of AD is the formation of b-amyloid ... b-amyloid (Ab) plaques, composedprimarily of Ab(1–40) and Ab(1–42) peptides, whichassociate to form abnormal extracellular deposits of ﬁbrils and amorphous aggregates [36]. Ab is a metallo-protein...

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