Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx

... sub-strate and quantiﬁed by using the DE- 81 ﬁlter paperS103Y 112 R207 /19 9K173 /16 5G145/D137D142 /13 4E1 41/ 133L75/67D2 01/ 193G77/69G197 /18 9G145/D137ABFig. 2. Superposition of PRTFDC1 with ... 10 6± 1. 0 · 10 6 (10 0%)GuaninePRTFDC1 36 .1 ± 14 .3 2.9 ± 0.7 1. 36 ± 0.34 3.9 · 10 4± 9.3 · 10 3(0.09%)HPRT 9.9 ± 0.2 899 ± 11 7 406 ± 53 4.5 · 10 7± 1. 0 · 10 7 (10 0%)M. Welin et al. Studies of ... (µmol·min 1 ·mg 1 )V (µmol·min 1 ·mg 1 )0.20 .1 0.00 50 10 0 15 0–50200400600800 10 000 50 10 015 0 200 250S–50 02468 10 12 50 10 015 0 200 250SS/VS/V0 10 0 .10 0.080.060.040.02 10 20 30...

Tài liệu Báo cáo khoa học: Structural and functional studies on a mesophilic stationary phase survival protein (Sur E) from Salmonella typhimurium ppt

... 77 61 55 β3 β4 α2 1 α3 β6 α4 β7 13 β8 α5 12 η2 η3 α6 1 β9 10 11 β2 1 β5 11 1 Active site 12 0 14 7 15 5 227 17 3 213 247 234205 19 9 19 5 2 1 5 5 12 8 13 9 10 11 ABCFig. ... <Ihj> is the jth observation of reﬂection h and <Ih> is its mean intensity.2 1 3 4 3 1 6 18 9 18 5 17 9 17 8 207 16 7 16 5 10 3 98 93 6 14 34 68 64 52 10 5 12 5 13 1 16 0 222 17 1 218 86 ... characterization of the protein The purity and molecular mass of the protein were checkedby 12 % SDS ⁄ PAGE and MALDI-TOF MS. Gel-permeationchromatography with 200 lLofa1mgÆmL )1 protein solu-tion...

Tài liệu Báo cáo khoa học: Structural and functional investigations of Ureaplasma parvum UMP kinase – a potential antibacterial drug target ppt

... KiCABBAABE204E204T197T197ACF133F133T1 31 T1 31 ACDEAK102D104K102D104D104K102CEDFig. 3. (A) Interactions between subunits A, B and C. (B) Hydrophobic interaction between the a4 helices ... 0.05 and 0.85 ± 0.05, respectively (Fig. 9).At 1 mm ATP, the K0.5,app(UMP) and Vmax,appvaluesfor F133N were 11 00 ± 15 0 lm and 10 7 ± 15 lmolÆmin )1 Æmg )1 , respectively. For F133A, the ... Phe133 of UpUMPK wasmutated to Asn or Ala. The mutant enzymes, F133N and F133A, were expressed, puriﬁed and characterized.With 1 mm UMP and ATP as substrates, the activities of F133N and F133A...

Tài liệu Báo cáo khoa học: Structural and biochemical characterization of a human adenovirus 2/12 penton base chimera pptx

... revised 11 July2006, accepted 27 July 2006)doi :10 .11 11/ j .17 42-4658.2006.05430.x The vertex of the adenoviral capsid is formed by the penton, a complex of two proteins, the pentameric penton base and ... clathrin-coated pits and endosomes [14 ,16 ]. It is thought that upon integrinbinding and before endocytosis, the fiber protein isshed from the virus [13 ,17 19 ]. One hypothesis for the mechanism of fiber removal ... 4339 containing the RGD motif, is located at the top of the distal domain within the first insertion, and faces the solvent-exposed exterior of the particle. As in the hAd2pb structures, even the...

Tài liệu Báo cáo khoa học: Structural and mechanistic aspects of ﬂavoproteins: electron transfer through the nitric oxide synthase ﬂavoprotein domain pdf

... hinge domains The composition of the two hinges that connect the FMN subdomain in NOS enzymes (H1 and H2 inFig. 5) defines the allowable movements of the FMNsubdomain and thus controls the FMN–NOSoxy ... 4 419 5, USAFax: 1 216 636 010 4Tel: +1 216 445 6950E-mail: stuehrd@ccf.org(Received 13 March 2009, revised 18 May2009, accepted 28 May 2009)doi :10 .11 11/ j .17 42-4658.2009.0 712 0.xNitric oxide ... [28 ,10 2 ,10 5 ,11 5]b-finger (e ⁄ n ⁄ iNOS) ? ? ND ND ND ? [34,35]R1229E nNOS ›b=b›› ND fl [77]F1395S nNOS ›b=b› = flfl[ 61, 72 ,11 6]R1400E nNOS ›b›b›fl › fl [60]S1 412 D nNOS ›› ›= ›fl[88]S 117 9D...

Tài liệu Báo cáo khoa học: Structural and mechanistic aspects of ﬂavoproteins: probes of hydrogen tunnelling pptx

... 2.76a0. 51 ± 0.04 [15 ,18 ]MR b-NADH 6.8 ± 0 .1 a7.4 ± 1. 5a0 .12 ± 0.09 [15 ,18 ]MR cylohexen -1- one 3.5 ± 0.2 )0.5 ± 1. 8 4 ± 2 [15 ]GO 2-deoxyglucose $10 b$0b $10 g[73]CO choline 10 .6 ± 0.6b 1. 0 ... Manchester M1 7ND, UKFax: +44 16 1 306 8 918 Tel: +44 16 1 306 515 2E-mail: nigel.scrutton@manchester.ac.uk(Received 23 December 2008, revised 28April 2009, accepted 1 May 2009)doi :10 .11 11/ j .17 42-4658.2009.0 712 1.xAt ... both MR and PETNR, at room temperature, kon, the apparent rate of coenzyme binding is $10 6m )1 Æs )1 . The rate of NAD(P)H dissociation, koff,is $10 2s )1 and the apparent rate of H transfer,...

Tài liệu Báo cáo khoa học: Structural and mechanistic aspects of ﬂavoproteins: photosynthetic electron transfer from photosystem I to NADP+ doc

... hydride transfer [11 4– 11 7 ,11 9]. S223 and Y235 at the AnFNR 2¢P-AMP siteare critical in determining the specificity and efficientcoenzyme orientation [99 ,11 5 ,12 1]. The 15 5 16 0 and 2 61 268 loops, which ... affinity and selectivity [11 7 ,11 8 ,12 2 12 6]. Structural studies have allowed us to postulate astepwise mechanism in which the nucleotide must bindto a bipartite site [59,62 ,11 4 ,11 7 ,12 7]. The first ... Role of neighboringFMN side chains in the modulation of flavin reductionpotentials and in the energetics of the FMN:apoproteininteraction in Anabaena flavodoxin. Biochemistry 43, 15 111 15 1 21. 27...

Tài liệu Báo cáo khoa học: Structural and functional speciﬁcities of PDGF-C and PDGF-D, the novel members of the platelet-derived growth factors family docx

... aFig. 2. The CUB domain. The CUB domains of PDGF-C and PDGF-D aligned with the prototype CUB domains of human neuropilin (acces-sion no CAI402 51) and human bone morphogenic protein- 1 (BMP -1, accession ... urchin EGF-like protein and bone morphogenic protein 1 [16 ]. These proteins areoften referred to as the prototype CUB domains. Like the prototype CUB domains, the PDGF-C and -DCUB domains span ... cancerOvarian cancerPDGF-D(98,99 ,10 0 ,10 1)(82,94)(30,80, 81) (85 ,11 0)(82,89)Lung (67,84), Kidney (30,85)Pancreas (10 8), Heart (8,38,67 ,10 9)Liver (10 6 ,11 2)Liver (80 ,11 0 ,11 2)Kidney (85)(89)(89)(44,66)PDGF-CFig....

Tài liệu Báo cáo khoa học: Structure and membrane interaction of the internal fusion peptide of avian sarcoma leukosis virus pdf

... IFP–F13 IFP–wt IFP–F13Secondary structure percentagea-Helix 57 ± 3 66 ± 3 60 ± 4 64 ± 3Disorder 12 ± 2 11 ± 3 12 ± 3 11 ± 3b-Sheet 24 ± 1 20 ± 2 22 ± 1 22 ± 1 b-Turn 7 ± 1 3 ± 2 6 ± 1 3 ± 1 Helix ... in the region 10 18 and 20–26. (Lower) Helical segments can b e observed i n 1 11 a nd 14 –28 bycontiguous (i,i +3) interactions. The absence of (i,i +3) cross-peaksbetween L eu 11 and Gly14 ... residues 16 18 at the aqueous boundary at acidic p H:oligomerization and the c onformational ﬂexibility. J. Biol. Chem.275, 19 150 19 158. 16 . Johnso n, W.C. (19 90) Protein secondary structu re and...

Tài liệu Báo cáo khoa học: Bioinformatic and enzymatic characterization of the MAPEG superfamily doc

... 29–33) and C (position 35) ascompared to GenBank AF 111 246 [ 61] . In the EST clone, the asparagine at position 42 of GenBank AF 111 246wasreplaced with a lysine. The cDNA was digested with NdeI and ... activity(lmolÆmin )1 Æmg )1 )CuOOH GPxactivity(lmolÆmin )1 Æmg )1 )Activity of purified enzyme Human MGST1 [ 71, 72] 1. 9 0.04Rat MGST1 [43] 2 0.08Xenopus laevis, frog [18 ] 210 2 .1 Esox lucius, ... 8% of the E. coli membrane asso-ciated proteins. The specific activity of the partiallypurified enzyme with 1- chloro-2,4-dinitrobenzene was 11 ± 0.4 lmolÆmin )1 Æmg )1 (mean ± SD, n ¼ 3). The activity...

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