Tài liệu Báo cáo khoa học: Homologous expression of the nrdF gene of Corynebacterium ammoniagenes strain ATCC 6872 generates a manganese-metallocofactor (R2F) and a stable tyrosyl radical (Y•) involved in ribonucleotide reduction ppt
... 2010 The Authors Journal compilation ª 2010 FEBS
Homologous expression of the nrdF gene of
Corynebacterium ammoniagenes strain ATCC 6872
generates a manganese-metallocofactor (R2F) and a stable
tyrosyl ... & Auling G (1998) Clon-
ing and sequencing of the nrdF gene of Corynebacterium
ammoniagenes ATCC 6872 encoding the func...
... molecular basis for new therapeutic strate-
gies targeting specific pathways to treat human heart
disease.
Materials and methods
Animals
Neonatal Wistar rats (1-3 days) were purchased from the
Animal ... kDa
nucleolin ⁄ C23 fragment accompanied by the appear-
ance and an increase in the 80 kDa fragment (Fig. 2).
Although both the untransfected cells and the cells
transfected...
... Ca
2+
-dependent
manner almost as effectively as intact Akazara scallop troponin. Therefore,
Akazara scallop troponin regulates the contraction through the activating
mechanisms that involve the region spanning ... of both TnC and Ca
2+
; lanes b and e, in the presence of TnC and the absence of Ca
2+
; lanes c and f, in the presence of both
TnC and Ca
2+
. Ac...
... four
types of features using the event of “Abdul Halim
Khaddam resigns as Vice President of Syria” and the
sentence The resignation of Khaddam was abrupt”
as an example. In particular, the “depth” features ... de-
noting the index of the word that is the descendant
in d
x
and d
x
.a denoting the ancestor. We define the
following matching function to match...
... change in path C,
and enabling a conformational change in path A, thereby
increasing the rate of both cycles.
In reaction path A, binding of Mg
2+
to subunit 2 induces
a conformational change from ... Dimer asymmetry and the catalytic cycle of alkaline phosphatase
from
Escherichia coli
Stjepan Orhanovic
´
and Maja Pavela-Vranc
ˇ
ic
ˇ
Department of Chemistry, Facu...
... a DNA-binding domain, a ligand-binding
domain and a transactivation domain. The DNA-bind-
ing domain is responsible for DNA binding specificity
and dimerization, and the ligand-binding domain is
responsible ... eukaryotes indicate that
they have crucial and distinct roles in gene activation
and other cellular events. Although the discovery of
MLLs and characteriza...
... 5¢-GGGCACTC
AGCCAGGGGACATCCTGCCCAA-3¢ as forward and
5¢-GATACAAGTTTGCACACCTTTGCACTTCTG-3¢ as
reverse [58]. The PCR amplification was performed in a
total volume of 50 lL reaction mix containing 1 ... concentration and qual-
ity, the GAPDH gene was also amplified by using a similar
protocol with the housekeeping gene specific primers:
5¢-CCATGGAGAAGGCTGGGG-3¢ as forward and...