... 5¢-CCTGCCGAGCTCCTATTACACAACGCCACCAACCATCAG-3¢. The PCR solution was prepared in the buffer supplied with the enzyme, and contained Aba, Abb and Abcat40 nm each, and the start and stop primers Abstart and Abstop at 600 nm each, and 200 ... acarbon-coated formvar grid, left for 1 min, fixed with glu-taraldehye, wicked dry with filter paper, and 2% uranylacetate was added and the mixture was incubated for 2 min. The grid was wicked dry and ... reverse-phase HPLC. For both synthetic and recombinant Ab peptides, the correct mass was confirmed by MALDI-TOF MS and LC-MS.PCR and cloning procedureSynthetic genes for Ab(M1–40) and Ab(M1–42) weredesigned...