Tài liệu về " Glycoprotein methods protocols - biotechnology " 15 kết quả
Glycoprotein methods protocols - biotechnology 048-9-001-013.pdf
Glycoprotein methods protocols - biotechnology ... different methods are available: solution assays such as colorimetric assays for hexose and sialic acid; membrane-based methods such as slot-blotting and staining with periodic acid-Schiff reagent;... correct volume of 10 Davies and Carlstedt ice-cold dry propan-1-ol to give a 100 mM solution DFP is unstable in water but can be stored at –20°C in ...
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Glycoprotein methods protocols - biotechnology 048-9-027-044.pdf
Glycoprotein methods protocols - biotechnology ... to T-antigen (β-d-Gal 1-3 GalNAc), but also to structures found within the backbone of oligosaccharides (β-d-Gal 1-3 / 4GlcNAc) (19) Demonstration of PNA binding is not necessarily evidence of T-antigen... Blue 8GX in 0.1 N HCl (pH 1.0) HID: Dissolve 120 mg of N,N-dimethyl-m-phenylenediamine dihydrochloride (Sigma) and 20 mg of ...
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Glycoprotein methods protocols - biotechnology 048-9-045-055.pdf
Glycoprotein methods protocols - biotechnology . 3,3'-diaminobenzidine, 4-chloronapthol mix forHRP [14]; 5-bromo-4-chloro-3'-indolyphosphate toluidine salt, nitro blue tetrazolium chlo-ride mix. (v/v) Nonidet P-40 or Tween-20.4. Enzyme substrates: 2,2'-azino-bis(3-ethylbenzathiazaline 6-sulfonic acid) (ABTS) (1 mg/mL,A405nm), O-phenyldiamine
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Glycoprotein methods protocols - biotechnology 048-9-057-064.pdf
Glycoprotein methods protocols - biotechnology . Ross-Murphy S. B. (1988) Mucus glycoproteingels: role of glycoprotein polymeric structure and carbohydrate side-chains in gel-forma-tion. Carbohydrate Res. 178,. water-soluble mounting prepared by dissolving 10 g of gelatin in 60 mL ofdistilled water mixed with 250 mg of phenol in 70 mL of glycerol.8. Poly-L-lysine-coated
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Glycoprotein methods protocols - biotechnology 048-9-065-073.pdf
Glycoprotein methods protocols - biotechnology . medium byWestern-type dot-blot procedure. Spot aliquots of the homogenates or media on nitrocel-lulose paper, using the dot-blot apparatus and air-dry for 5. present in the rough endoplasmic reticu-lum, containing N-glycosylation but no O-glycosylation (which occurs only after arri-val in the Golgi apparatus) (1,8).
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Glycoprotein methods protocols - biotechnology 048-9-075-085.pdf
Glycoprotein methods protocols - biotechnology . assessment of pep-tide homogeneity is advisable. We analyze aliquots of the peaks by using matrix-assistedlaser desorption ionization time-of-flight mass spectrometry. contained a complex mixture of mucin-type glycoproteins.However, until recently and with the advent of the new mucin-specific probes arisingfrom cDNA cloning
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Glycoprotein methods protocols - biotechnology 048-9-087-096.pdf
Glycoprotein methods protocols - biotechnology . Heterogeneity and Size Distribution of Gel-Forming Mucins 8787From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The MucinsEdited by:. is rate-zonalcentrifugation, which separates molecules not on hydrodynamic volume alone but ontheir mass-to-volume ratio as well. The basis of rate-zonal
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Glycoprotein methods protocols - biotechnology 048-9-111-119.pdf
Glycoprotein methods protocols - biotechnology . Amino Acid Analysis of Mucins 113113From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The MucinsEdited by: A. Corfield. and Gooley, A. A. (1999) 2-DE spots amino acid analysis with9-fluorenylmethyl chloroformate, 2-D Protein Gel Electrophoresis Protocols (Link, A. J.,ed.),
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Glycoprotein methods protocols - biotechnology 048-9-121-128.pdf
Glycoprotein methods protocols - biotechnology . profiles of PTH-threonine-GalNAc-Gal (black arrow-heads) and PTH-threonine-GalNAc (gray arrowheads) from the Applied Biosystems Procise. Stan-dard amino acids. glycosylated amino acids released from Edman degrada-tion: the identification of Xaa-Pro-Xaa-Xaa as a motif for Thr-O-glycosylation. Biochem.Biophys. Res. Commun.
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Glycoprotein methods protocols - biotechnology 048-9-129-141.pdf
Glycoprotein methods protocols - biotechnology . chain protecting group Amino acidt-Butyl ether S, T, Yt-Butyl ester D, Et-Butoxycarbonyl K, H, W2,2,5,7,8-Pentamethylchroman-6-sulfonyl RTrityl CSynthetic. monohydratemade up to 1 L with distilled water, pH 4.0.5. 2,2'-azino-bis[3-ethylbenz-thiazoline-6-sulfonic acid] (ABTS) substrate solution (Sigma):0.5 mg/mL
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Glycoprotein methods protocols - biotechnology 048-9-143-155.pdf
Glycoprotein methods protocols - biotechnology . benzyl 2-acetamido-2-deoxy-α-D-galactopyranoside (benzyl-α-GalNAc) (Sigma),filter sterilized.3. Peptide-N 4-( acetyl-ß-glycosaminyl) asparagine amidase (N-glycosidase. addition of 6.6 mg of 4-nitro blue tetrazolium chloride and 1.65 mg of 5-bromo-4-chloro-3-indolyl-phosphate), and stop solution (10 mM Tris-HCl, 1 mM EDTA, pH
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Glycoprotein methods protocols - biotechnology 048-9-159-180.pdf
Glycoprotein methods protocols - biotechnology . 250 mm). 1, PA-Gal; 2, PA-Glc; 3, PA-Man; 4, PA-Rib; 5, PA-Fuc; 6, PA-Rham; 7, PA-ManNAc; 8, PA-deoxy-Rib; 9, PA-GlcNAz; 10, PA-GalNAc; 11, PA-NeuAc.Monosaccharide. CaponDifferent chromophores such as 2-aminopyridine (20), 8-aminonaphthalene-1,3,6-trisulfonic acid (ANTS) (21), ethyl-4-aminobenzoate, and 4-aminobenzonitrile (22),have
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Glycoprotein methods protocols - biotechnology 048-9-181-190.pdf
Glycoprotein methods protocols - biotechnology . by normal- or re-versed-phase high-performance liquid chromatography (HPLC) (8) or anion-exchange chro-matography, e.g., high-performance anion-exchange. HPAEC Analysis of O-Glycanase-Released Galβ 1-3 GalNAc1. Inject a 1- g aliquot of the O-Glycanase-released product, containing 320 ng of D-meli-biose as internal
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Glycoprotein methods protocols - biotechnology 048-9-191-209.pdf
Glycoprotein methods protocols - biotechnology . GalNAc-ol H-2, H-4, H-5 and H-6'Fig. 1Analysis of Mucin-Type O-Linked Oligosaccharides 193atoms; Gal H-3 and H-4 atoms; Fuc H-5 and H-6 atoms; NeuAc H-3ax. the H-4 resonance. Relayed COSY spectra have the advan-tage of successively assigning the H-2 (COSY), H-3 (one-step-relayed COSY), and H-4 (two-steps-relayed
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Glycoprotein methods protocols - biotechnology 048-9-211-216.pdf
Glycoprotein methods protocols - biotechnology . UK).5. 1 mM 2-sulfo-N-acetylglucosamine, 3-sulfo-N-acetylglucosamine, and 6-sulfo-N-acetylglucosamine, in water (see Note 3).2.2. Equipment1. High-performance. (linkage lability: 6- > 2- > 3-) , we recommend including two external controls that contain an equimolar mix-ture of 2-, 3- and 6-sulfo-N-acetylglucosamine
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